COMPARISON OF FGF -8, FGF -10, FGF- RECEPTOR 2,
ANDROGEN RECEPTOR, ESTROGEN RECEPTOR-Α
AND SS IN HEALTHY AND HYPOSPADIAC CHILDREN
Emaratpardaz N, Turkyilmaz Z, Karabulut R, Dayanir D, Kaya C, Sert AAE, Arkan G, Ucaner FA, Kapisiz A, Eryilmaz S, Atan A, Sonmez K
*Corresponding Author: Prof. Ramazan Karabulut, MD, Gazi University Faculty of Medicine,
Department of Pediatric Surgery, Besevler, 06550, Ankara-Turkey. Tel: +90 312 2026210;
Fax: +90 312 2230528. E-mail: karabulutr@yahoo.com, ramazank@gazi.edu.tr
page: 21
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RESULTS
The study was carried out with a total of 40 children.
The children’s ages ranged from 14 months to 12 years
with a mean age of 65.92±33.20 months. The mean age
in the control group was 59.40±31.80 months (12-108
months) and the mean age in the hypospadias group was
72.45±34.09 (14-132 months). Of the patients with hy-
pospadias, 2 had coronal, 11 had subcoronal and 7 had
mid-penile hypospadias. In the H&E stained sections
of the preputial tissues obtained from both groups, the
epidermis was observed to be consistent with the fea-
tures of the stratified squamous epithelium. There was
no significant histomorphological differences between
two groups (p>0.05).
IHC uptake of AR, ER- α, ER- β markers was eval-
uated with the quantitative scoring system of stratified
squamous epithelium [9]. While the score was (+) in 18
patients in the AR control group, the score was (+++) in 15
patients with hypospadias and (++) in 5 patients. Similarly,
ER-α and ER-β markers were detected in a larger area in
the epidermis in the hypospadias group (Table 2), (Fig. 1,
2 and 3). When the AR and ER receptors were scored, it
was shown that the foreskin of children with hypospadias
contained statistically higher AR and ERs than the foreskin
of children without hypospadias (Table 4) (p<0.05).
The levels of FGFR-2, FGF-8, FGF-10 in epidermis
and dermis were quantitatively evaluated. Th involve-
ment pattern was classified as 1, 2, 3 for epidermis and
classified as A, B, C for dermis. When the epidermis
area was examined, Pattern 3 involvement was observed predominantly in control patients, while Pattern 1 in-
volvement was observed in patients with hypospadias.
When the dermis areas were examined, it was observed
that 17 of the patients in the control group of FGFR-2
had Pattern C, 3 had Pattern B, 19 patients with hypo-
spadias had Pattern B and 1 had Pattern A involvement
(Fig. 4). If FGF-8, 6 Pattern C and 14 Pattern B uptake
Figure 4. In the control group, FGFR-2 uptake was observed in more than 50% of Keratinocytes (Epidermis Pattern 3) and clustered
positive cells in more than 50% of all visible dermal cells (Dermis Pattern C). Limited to basal involvement in hypospadias group
(Epidermis Pattern 1) and involvement of less than 50% of dermal cells (Dermis Pattern B) (IHC Staining, 200 X).
Figure 5. In the control Group, more than 50% of keratinocytes were involved (Epidermis Pattern 3) and less than 50% of dermal cells
are involved (Dermis Pattern B) for FGF-8. In the hypospadias group, it was limited to basal involvement (Epidermis Pattern 1) and
involvement of less than 50% of dermal cells (Dermis Pattern B) (IHC Staining, 200 X).
were detected in control patients, while 17 Pattern B and
3 Pattern A uptake were detected in hypospadias patients
(Fig.5). It was Pattern C in 17 of the FGF-10 control
patients, Pattern B in 3 of the patients with hypospadias,
it was observed as Pattern B in 18 of the patients with
hypospadias and as Pattern A in 2 of the patients with
hypospadias (Fig.6), (Table 3). There was a higher rate of uptake in both epidermis
and dermis areas for FGFR-2, FGF-8 and FGF-10 in the
control group. There was a statistically significant differ-
ence between two groups (p<0.05) (Table 4).
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