Balkan Journal of Medical Genetics

THE PREDISPOSITION FOR TYPE 2 DIABETES MELLITUS AND METABOLIC SYNDROME
Zenoaga-Barbăroșie C, Berca L, Vassu-Dimov T, Toma M, Nica MI, Alexiu-Toma OA, Ciornei C, Albu A, Nica S, Nistor C, Nica R
*Corresponding Author: Corresponding Author: Ass. Prof. Silvia Nica MD, PhD, Bucharest Emergency University Hospital, Splaiul Independentei Street, no 169, 5 District, Bucharest, Romania, 050098 Tel.: +40-21-31880500, email: silvia.nica@umfcd.ro
page: 21

MATERIAL AND METHODS

Clinical data The case-control study included Caucasian men considered healthy (n=120) or diagnosed with T2DM (n=120) or MetS (n=75). The American Diabetes Association 2016 and NCEP ATP III criteria were used for the diagnosis of these diseases (17). Healthy individuals were selected based on a standard clinical evaluation and on paraclinical data. Patients with a diagnosis of chronic kidney diseases, retinopathy, diabetic peripheral neuropathy or with an addiction to drugs were not included in the study. We collected clinical, paraclinical, and lifestyle data from all patients. Subjects were considered smokers if they smoked between 2 and 25 cigarettes per day for at least a year. Alcohol consumers were considered those who drunk at most 50 g alcohol per day for at least a year, but were not heavy drinkers (56g alcohol/day). The research was approved by the ethics committee of the National Research and Development Institute for Food Bioresources (966/27.08.2019). Research methods Genomic DNA was extracted from peripheral venous blood using the Promega Wizard Genomic DNA purification kit (Promega Corporation, Madison, WI), followed by a Polymerase Chain Reaction (PCR) to genotype the rs4646994 (ACE I/D) (18), rs1799983 (eNOS VNTR 4a/b) (18), and rs53576 (OXTR A>G) (19) polymorphisms. A PCR-restriction fragment length polymorphism was used to genotype the ATR1 rs5186 (A1166C) (18), CAT rs7943316 (-21A/T) (20), and SOD1 rs2234694 (+35A/C) polymorphismes (20). Statistical analysis Statistical analysis was performed with the MedCalc software (version 20.111, Ostend, Belgium). After Bonferroni correction for multiple hypotheses (n=33) a p value at p<0.0015 was considered statistically significant.

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