SINGLE NUCLEOTIDE POLYMORPHISMS IN IL-1A
RS1800587, IL-1B RS1143634 AND VITAMIN D RECEPTOR
RS731236 IN STAGE III GRADE B/C PERIODONTITIS
Özturk Özener H.1, Tacal Aslan B.2, Eken B.F.2, Agrali Ö.B.1,
Yildrim H.S.1, Altunok E.Ç.3, Ulucan K.2, Kuru L.1
*Corresponding Author: Assist. Prof. Dr. Hafize Öztürk Özener, Address: Marmara University, Faculty
of Dentistry, Department of Periodontology Basıbuyuk, Maltepe, Istanbul, Turkey. Fax number: +90 216
421 02 21; E-mail: hafize.ozturk@marmara.edu.tr
page: 10
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RESULTS
Table 1 demonstrates demographic data and clinical
features of the periodontitis patients and the healthy
subjects. All periodontal clinical parameters in the patients
with periodontitis were significantly higher comparison to
the healthy individuals, as expected (p<0.000).
In Table 2, the distributions of polymorphisms in all
study populations agreed to the Hardy–Weinberg equilibrium
(p > 0.05), except IL-1B (rs1143634) in the healthy
group (X2=7.75; p < 0.05). Comparisons of genotype frequencies
between the periodontitis patients and healthy
individuals are presented in Table 2. IL-1A (rs1800587)
and IL-1B (rs1143634) polymorphisms did not show
any association with periodontitis (OR=1.142, p=0.793,
OR=1.726, p=0.371, respectively). Considering the VDR
TaqI gene polymorphism, the frequencies of TT genotype
in the periodontitis and healthy subjects were 36% and
49%, respectively, and CC genotype 19% and 10%, respectively.
The results showed that the genotype CC was
associated with a 2.586 times higher risk of periodontitis
with a statistically significant risk coefficient (p=0.031).
The allelic distributions of each gene polymorphisms
are presented in Table 3. Allele frequency regarding IL-1A
(rs1800587) demonstrated no significant differences between
the healthy individuals and the periodontitis patients.
The C allele in IL-1B (rs1143634) was detected more frequently
in healthy individuals than patients (84.5% versus
76.5% OR=1.167, p=0.045). In VDR TaqI gene polymorphism,
the C allele frequency in the periodontitis patients
(83%) was higher than in the healthy subjects (61%). The
C allele was statistically significant in the periodontitis patients
with an OR of 1.61 (95% CI= 1.071–2.441, p=0.022).
Model wise genotypic distributions regarding codominant,
dominant, recessive and overdominant models
are presented in Table 4. Model wise genotypic distribution
of IL-1A (rs1800587) did not demonstrate any association
with periodontitis (Table 4). Regarding the IL-1B
(rs1143634) polymorphic region, the frequency of CC
genotype was higher in healthy subjects and associated
with a decreased risk of periodontitis under dominant
model [OR (95% CI) =0.527 (0.289-0.960), p=0.035].
Likewise, VDR TaqI gene polymorphism showed alliance
with periodontitis, on the other hand, the dominant, recessive
and overdominant models did not demonstrate any
relation with periodontitis (Table 4).
Associations between different grades of periodontitis
and gene polymorphisms in IL-1A (rs1800587), IL-1B
(rs1143634) and VDR TaqI are demonstrated in Table 5.
Both allele and genotype frequency of IL-1A (rs1800587)
gene polymorphism did not show any association with
any grade of periodontitis (p>0.05). The C and T alleles
frequencies of IL-1B (rs1143634) polymorphism were
76 (74.5%) and 26 (25.5%) in the Grade B periodontitis,
77 (78.6%) and 21 (21.4%) in the Grade C periodontitis
and 169 (84.5%) and 31 (15.5%) in the healthy subjects,
respectively. The difference was found to be statistically
significant only between the Grade B periodontitis and
healthy subjects [OR=1.865; 95% CI (1.037-3.355),
p=0.036]. A comparison of the VDR TaqI genotypes and
alleles between the Grade B periodontitis and healthy subjects
revealed that the TT genotype, and T allele were more
frequently observed in the healthy subjects (TT vs. CC, TT
vs. CT, T vs. C, p˂0.05). In the evaluation of TaqI polymorphism
in Grade B and C periodontitis, individuals with
the CT genotype have a 3.207 times higher risk of Grade
B periodontitis than individuals with the TT genotype.
[CT vs. TT, OR (95% CI) =3.207 (1.286-7.997), p=0.011].
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