ASSOCIATIONS OF BIOCHEMICAL CHANGES AND
MATERNAL TRAITS WITH MUTATION 1843 (C>T)
IN THE RYR1 GENE AS A COMMON CAUSE
FOR PORCINE STRESS SYNDROME
Popovski ZT, Tanaskovska B, Miskoska-Milevska E, Andonov S, Domazetovska S
*Corresponding Author: Professor Zoran T. Popovski, Ph.D., Faculty of Agriculture and Food Sciences, Bld “Aleksandar
Makedonski,” bb PB 297, 1000 Skopje, Republic of Macedonia. Tel: +389-70-252-731. Fax: +389-2-3134-310. E-mail:
zoran_popovski@yahoo.com
page: 75
|
|
DISCUSSION
We did not find a significant correlation between the
level of Ca2+ and stress susceptibility [12,19]. The values
of plasma Ca2+ showed no significant differences among the different PSS genotypes. All values for concentrations
of Ca2+ were in the reference range. The biochemical data
for total protein and ALT did not show significant association
with the PSS genotypes.
The potential link between creatinine and bilirubin
values with the PSS genotype has been previously presented
[20]. The levels of creatinine, bilirubin and AP
were in the frame of their reference ranges although the
PSS-susceptible animals had higher values of creatinine,
bilirubin and AP compared to those from stress-free and
heterozygous animals. Nevertheless, the differences in the
three groups of animals in some parameters were highly
significant. Taking into account the role of AP in energy
metabolism, creatinine as a final product of increased energy
level and the releasing of myoglobin as one of the
bilirubin precursors, those differences are explainable.
Lactate dehydrogenase was used as a biochemical
marker for the identification of stress susceptible animals
before development of molecular tools [12,16]. Our data
confirmed that only the values of the enzyme from non
induced stress-free animals (398.00 U/L) were in the frame
of reference values, while the values of heterozygous (Nn)
animals were slightly out of that range (655.00 U/L). The
values of stress-susceptible animals were 2-fold higher
(894.00 U/L) than the maximum values of reference range
(159.00-475.0 U/L) (p <0.01). These data were consistent
with the role of LDH in the metabolism of glucose in anaerobic
glycolysis in humans [20]. The possible reason for
that is the fact that even non induced stress is a reason for
a slower rate of lactate and CO2 production from glucose,
so the enzyme is accumulated in plasma [21].
Creatine phosphokinase was reported to be affected
in PSS animals [21]. Our results showed that the values
of the enzyme from stress-free animals (276.00 U/L) were
within the normal range, the CPK values from the heterozygous
pigs (591.00 U/L) were out of range, and the
values of stress-susceptible animals were higher (764.00
U/L) than the maximum values of the reference range.
Those differences matched with the PSS genotype were
highly significant (p <0.01). The mechanism of CPK activity
is in energy metabolism and it is highly released by
the stress susceptible animals. In the human population,
up to 70.0% of stress susceptible subjects show increased
muscle-specific CPK activity in plasma [22].
Deviation of AST values were observed in the animals
with the different PSS genotype. We observed the highest
deviation between the values we measured and the reference
values. Only the data of stress-free animals (29.20
U/L) were in the frame of reference values, the values of
heterozygous animals were higher (58.30 U/L), while the
values of the homozygous animals (125.50 U/L) were
3-fold higher than those in the reference range (17.00-
45.00 U/L) (p <0.01). This is the first published evidence
about a possible association between AST and PSS.
Regarding maternal traits, we found that the heterozygous
PSS sows with genotype Nn expresses the best
reproductive ability, the highest number of newborn and
the lowest number of stillborn piglets compared with normal
(NN) and stress susceptible sows (nn) (p <0.05). Also,
in the Nn genotype, the number of weaned piglets was
the highest compared with the other genotypes (NN and
nn), but these differences were not statistically significant
(p >0.05). The lowest reproductive ability was observed
in the nn genotype, with the lowest number of newborn,
the highest number of stillborn and the lowest number
of weaned piglets. It is important to point out that the nn
category was represented by a limited number of samples,
and therefore, this might be due to a sampling effect. There
is no data about the association of maternal traits with
genotype for stress syndrome in humans, bearing in mind
that the same mutation occurs in the ryr1 gene in the human
and swine genome.
|
|
|
|
 |
Number 27
VOL. 27 (2), 2024 |
Number 27
VOL. 27 (1), 2024 |
Number 26
Number 26 VOL. 26(2), 2023 All in one |
Number 26
VOL. 26(2), 2023 |
Number 26
VOL. 26, 2023 Supplement |
Number 26
VOL. 26(1), 2023 |
Number 25
VOL. 25(2), 2022 |
Number 25
VOL. 25 (1), 2022 |
Number 24
VOL. 24(2), 2021 |
Number 24
VOL. 24(1), 2021 |
Number 23
VOL. 23(2), 2020 |
Number 22
VOL. 22(2), 2019 |
Number 22
VOL. 22(1), 2019 |
Number 22
VOL. 22, 2019 Supplement |
Number 21
VOL. 21(2), 2018 |
Number 21
VOL. 21 (1), 2018 |
Number 21
VOL. 21, 2018 Supplement |
Number 20
VOL. 20 (2), 2017 |
Number 20
VOL. 20 (1), 2017 |
Number 19
VOL. 19 (2), 2016 |
Number 19
VOL. 19 (1), 2016 |
Number 18
VOL. 18 (2), 2015 |
Number 18
VOL. 18 (1), 2015 |
Number 17
VOL. 17 (2), 2014 |
Number 17
VOL. 17 (1), 2014 |
Number 16
VOL. 16 (2), 2013 |
Number 16
VOL. 16 (1), 2013 |
Number 15
VOL. 15 (2), 2012 |
Number 15
VOL. 15, 2012 Supplement |
Number 15
Vol. 15 (1), 2012 |
Number 14
14 - Vol. 14 (2), 2011 |
Number 14
The 9th Balkan Congress of Medical Genetics |
Number 14
14 - Vol. 14 (1), 2011 |
Number 13
Vol. 13 (2), 2010 |
Number 13
Vol.13 (1), 2010 |
Number 12
Vol.12 (2), 2009 |
Number 12
Vol.12 (1), 2009 |
Number 11
Vol.11 (2),2008 |
Number 11
Vol.11 (1),2008 |
Number 10
Vol.10 (2), 2007 |
Number 10
10 (1),2007 |
Number 9
1&2, 2006 |
Number 9
3&4, 2006 |
Number 8
1&2, 2005 |
Number 8
3&4, 2004 |
Number 7
1&2, 2004 |
Number 6
3&4, 2003 |
Number 6
1&2, 2003 |
Number 5
3&4, 2002 |
Number 5
1&2, 2002 |
Number 4
Vol.3 (4), 2000 |
Number 4
Vol.2 (4), 1999 |
Number 4
Vol.1 (4), 1998 |
Number 4
3&4, 2001 |
Number 4
1&2, 2001 |
Number 3
Vol.3 (3), 2000 |
Number 3
Vol.2 (3), 1999 |
Number 3
Vol.1 (3), 1998 |
Number 2
Vol.3(2), 2000 |
Number 2
Vol.1 (2), 1998 |
Number 2
Vol.2 (2), 1999 |
Number 1
Vol.3 (1), 2000 |
Number 1
Vol.2 (1), 1999 |
Number 1
Vol.1 (1), 1998 |
|
|
