ASSOCIATIONS OF BIOCHEMICAL CHANGES AND MATERNAL TRAITS WITH MUTATION 1843 (C>T) IN THE RYR1 GENE AS A COMMON CAUSE FOR PORCINE STRESS SYNDROME
Popovski ZT, Tanaskovska B, Miskoska-Milevska E, Andonov S, Domazetovska S
*Corresponding Author: Professor Zoran T. Popovski, Ph.D., Faculty of Agriculture and Food Sciences, Bld “Aleksandar Makedonski,” bb PB 297, 1000 Skopje, Republic of Macedonia. Tel: +389-70-252-731. Fax: +389-2-3134-310. E-mail: zoran_popovski@yahoo.com
page: 75

MATERIALS AND METHODS

Blood was taken from 73 randomly chosen sows that were not exposed to stress. After the centrifugation step (1000 × g for 10 min. at room temperature), the plasma samples underwent further biochemical analyses, while the leucocytes served for DNA extraction. DNA extraction from leucocytes was done using a DNA purification kit (Cat. #A1125; Promega, Madison, WI, USA), according to the manufacturer’s instructions. Genotyping of PSS was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) by digestion of the amplified part of the ryr1 gene where the common 1843 (C>T) mutation occurs by digestion with HhaI restriction endonuclease, as previously described [18]. Biochemical analyses of nine biochemical parameters [bilirubin, Ca2+, creatinine, total protein, AP, aspartate aminotransferase (AST), alanine aminotransferase (ALT), CPK and lactate dehydrogenase (LDH)], were determined using an automatic analyzer (Cobas-Integra 700; Roche Diagnostics, Indianapolis, IN, USA). Quantitative records on newborn, stillborn and weaned piglets (piglets that became independent) required for the statistical analyses were obtained from the data kept on the farms. Statistical analysis for genotype distribution and allele frequencies were determined by individual and allele counting. For the quantitative parameters and productive traits, descriptive statistical parameters (mean, minimum, maximum and mean standard error (MSE) were calculated. Later, the differences in the genotypes were tested with the Student t-test. All statistical evaluations were performed using the Statistical Package for the Social Sciences (SPSS), version 6.1 for Windows Student Version (SPSS Inc, Chicago, IL, USA; 1994).



Number 19
VOL. 19 (2), 2016
Number 19
VOL. 19 (1), 2016
Number 18
VOL. 18 (2), 2015
Number 18
VOL. 18 (1), 2015
Number 17
VOL. 17 (2), 2014
Number 17
VOL. 17 (1), 2014
Number 16
VOL. 16 (2), 2013
Number 16
VOL. 16 (1), 2013
Number 15
VOL. 15 (2), 2012
Number 15
VOL. 15, 2012 Supplement
Number 15
Vol. 15 (1), 2012
Number 14
14 - Vol. 14 (2), 2011
Number 14
The 9th Balkan Congress of Medical Genetics
Number 14
14 - Vol. 14 (1), 2011
Number 13
Vol. 13 (2), 2010
Number 13
Vol.13 (1), 2010
Number 12
Vol.12 (2), 2009
Number 12
Vol.12 (1), 2009
Number 11
Vol.11 (2),2008
Number 11
Vol.11 (1),2008
Number 10
Vol.10 (2), 2007
Number 10
10 (1),2007
Number 9
1&2, 2006
Number 9
3&4, 2006
Number 8
1&2, 2005
Number 8
3&4, 2004
Number 7
1&2, 2004
Number 6
3&4, 2003
Number 6
1&2, 2003
Number 5
3&4, 2002
Number 5
1&2, 2002
Number 4
Vol.3 (4), 2000
Number 4
Vol.2 (4), 1999
Number 4
Vol.1 (4), 1998
Number 4
3&4, 2001
Number 4
1&2, 2001
Number 3
Vol.3 (3), 2000
Number 3
Vol.2 (3), 1999
Number 3
Vol.1 (3), 1998
Number 2
Vol.3(2), 2000
Number 2
Vol.1 (2), 1998
Number 2
Vol.2 (2), 1999
Number 1
Vol.3 (1), 2000
Number 1
Vol.2 (1), 1999
Number 1
Vol.1 (1), 1998

 

 


 About the journal ::: Editorial ::: Subscription ::: Information for authors ::: Contact
 Copyright © Balkan Journal of Medical Genetics 2006