ASSOCIATIONS OF BIOCHEMICAL CHANGES AND MATERNAL TRAITS WITH MUTATION 1843 (C>T) IN THE RYR1 GENE AS A COMMON CAUSE FOR PORCINE STRESS SYNDROME
Popovski ZT, Tanaskovska B, Miskoska-Milevska E, Andonov S, Domazetovska S
*Corresponding Author: Professor Zoran T. Popovski, Ph.D., Faculty of Agriculture and Food Sciences, Bld “Aleksandar Makedonski,” bb PB 297, 1000 Skopje, Republic of Macedonia. Tel: +389-70-252-731. Fax: +389-2-3134-310. E-mail: zoran_popovski@yahoo.com
page: 75

RESULTS

An experiment was conducted with sows genotyped for PSS using DNA based methods. Using a PCR-RFLP approach, we determined the genotypes for PSS in 73 DNA samples, identifying 60 stress-free animals (NN), 11 heterozygots (Nn) and two homozygous stress-susceptible animals (nn). Allele frequency of the N and n alleles was 0.897 and 0.103, respectively. The PCR-RFLP characterization of PSS is shown in Figure 1. The results from genotyping were matched with values of nine biochemical parameters that were examined as possible predictors of PSS susceptibility in non-stress induced animals (Table 1). The study also explored the relevance of different biochemical parameters that could be used as possible biochemical markers for PSS susceptibility. Maternity traits matched with the genotype for PSS are shown in Table 2.



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