ASSOCIATIONS OF BIOCHEMICAL CHANGES AND
MATERNAL TRAITS WITH MUTATION 1843 (C>T)
IN THE RYR1 GENE AS A COMMON CAUSE
FOR PORCINE STRESS SYNDROME
Popovski ZT, Tanaskovska B, Miskoska-Milevska E, Andonov S, Domazetovska S
*Corresponding Author: Professor Zoran T. Popovski, Ph.D., Faculty of Agriculture and Food Sciences, Bld “Aleksandar
Makedonski,” bb PB 297, 1000 Skopje, Republic of Macedonia. Tel: +389-70-252-731. Fax: +389-2-3134-310. E-mail:
zoran_popovski@yahoo.com
page: 75
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RESULTS
An experiment was conducted with sows genotyped
for PSS using DNA based methods. Using a PCR-RFLP
approach, we determined the genotypes for PSS in 73
DNA samples, identifying 60 stress-free animals (NN), 11 heterozygots (Nn) and two homozygous stress-susceptible
animals (nn). Allele frequency of the N and n alleles was
0.897 and 0.103, respectively. The PCR-RFLP characterization
of PSS is shown in Figure 1.
The results from genotyping were matched with values
of nine biochemical parameters that were examined
as possible predictors of PSS susceptibility in non-stress
induced animals (Table 1). The study also explored the
relevance of different biochemical parameters that could
be used as possible biochemical markers for PSS susceptibility.
Maternity traits matched with the genotype for PSS
are shown in Table 2.
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