Balkan Journal of Medical Genetics

POLYPLOIDY PHENOMENON AS A CAUSE OF EARLY MISCARRIAGES IN ABORTION MATERIALS
Yildirim ME, Karakus S, Kurtulgan HK, Ozer L, Celik SB
*Corresponding Author: Malik Ejder Yildirim, Associate Professor. Department of Medical Genetics, Faculty of Medicine, Sivas Cumhuriyet University, 58104 Sivas, Turkey. Phone: 03462581085, Email: nemalik2002@gmail.com
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MATERIALS AND METHODS

Sample selection After a comprehensive anamnesis for each case, ninety two abortion materials were included in this research and all were first trimester miscarriages. Samples were collected at Cumhuriyet University Research Hospital between 2017 and 2021. The study complies with the Helsinki Declaration and it was approved by the Ethics Committee of Cumhuriyet University. Informed consent was obtained from the families. The mean age of the females participating in the study was calculated. Laboratory analysis For the examination of abortion materials, separate cultures were prepared by placing the sample into two flasks, one containing a half Chang D and another with Amniomax which were then left to incubate in a CO2 incubator at 37 °C. When there was sufficient growth, 60 μL colchicine were added for every 5mL of culture, for 45 minutes. The growth medium was then removed and a fixative solution was added for 10 minutes at room temperature. This step was repeated twice and then the plates were prepared as usual and stained by G band. Captured cells by a computerized system (Applied Imaging, USA) were analyzed and karyotyped in the metaphase stage. The results were defined according to the International System for Human Cytogenetic Nomenclature (ISCN). A few cases with a family history of translocation or inadequate karyotype analysis were subsequently analyzed with aCGH for confirmation. Statistical analysis was performed with SPSS program, version 22.0 (SPSS, Chicago, IL, USA). For the evaluation of the data, descriptive statistical criteria such as frequency, mean and standard deviation were chosen. The Independent Sample T test was used to compare the means.

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