DETECTING EGFR MUTATIONS IN PATIENTS WITH NON-SMALL CELL LUNG CANCER
Hammoudeh ZA, Antonova O, Staneva R, Nikolova D, Kyuchukov Y, Penev A, Mintchev T, Koleva V, Hadjidekova S, Toncheva D
*Corresponding Author: Zora A. Hammoudeh, Molecular Biologist, Department of Medical Genetics, Medical University Sofia, 2 Zdrave Str., 1431 Sofia, Bulgaria. Tel: +359-2-917-2735. Mobile: +359-88-943-0505. E-mail: zorahammoudeh@yahoo.com
page: 13

RESULTS

The NSCLC samples were classified by an experienced pathologist into 50.1% (n = 276) adenocarcinoma and 49.9% (n = 275) other histological types. Distribution by stage was as follow: 38 patients were diagnosed with stage IIIB and 513 were stage IV. There were 338 smokers (61.3%) and 213 were non smokers (38.7%). The risk of having lung cancer for smokers increased with the number of cigarettes smoked per day or duration of smoking [10]. The clinical characteristics of the patients are shown in Table 1. Mutational status was determined according to the manufacturer’s protocol. All samples analyzed had an internal control CT value in the reference range 23-30, 69. Positive amplification for EGFR mutational assay was considered in the case of CT value within the range of 15-40. The ΔCT value for each mutation sample showing positive amplification was calculated by the formula: ΔCT = mutation CT-control CT and compared to the reference provided by the manufacturer (Figure 1). The EGFR gene mutations were found in 10.0% of the NSCLC samples (n = 55) using the therascreen EGFR assay (Qiagen Ltd.) (Table 2).



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