PREVALENCE OF CHROMOSOMAL ABNORMALITIES IN INFERTILE COUPLES IN ROMANIA
Mierla D1,*, Malageanu M1, Tulin R1,2, Albu D1,2
*Corresponding Author: Dana Mierla, PhD, Department of Genetics, Life Memorial Hospital, Grivita Street, Bucharest, Romania. Tel: +40-72-147-9083. E-mail: dana_mierla@yahoo.com
page: 23

MATERIALS AND METHODS

This is a retrospective study, performed at the Department of Reproductive Medicine, Life Memorial Hospital, Bucharest, Romania. In this study, cytogenetic analyses were performed on a population of 2195 patients with reproductive failures who were referred to our clinic for investigation between August 2007 and December 2011. The control group consisted of 87 fertile persons with one or two previous pregnancies (Table 1). Informed consent was obtained from the patients and controls prior to collection of blood samples with heparin as anticoagulant. The blood samples were grown in culture medium (PB Max and chromosome B medium; Gibco/ Invitrogen, Grand Island, NY, USA) for 72 hours, and processed using GTG banding techniques. For each case, 10 metaphases were analyzed in accordance with the International System for Human Cytogenetic Nomenclature (ISCN) 2013 [17]. The banding resolution was 400-550 band per haploid set (BPHS). Molecular cytogenetic methods were also used [metaphase and interphase fluorescent in situ hybridization (FISH)]. Statistical Analysis. The results of the two groups were compared using the two-tailed Fisher’s exact test and calculated with GraphPad Software (http://www.graphpad .com/quickcalcs/contingency1. cfm8).



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