APOPTOSIS GENE EXPRESSION PROFILE IN EARLY-STAGE NON SMALL CELL LUNG CANCER
Metodieva SN1, Cherneva RV, Nikolova DN, Genchev GD, Petrov DB, Toncheva DI
*Corresponding Author: Svetlana Nikolova Metodieva, Department of Medical Genetics, Medical University Sofia, 2 Zdrave str, 1431 Sofia, Bulgaria; Tel.: +359-2-952-0357; Fax: +359- 2-952-0357; E-mail: svetlana.metodieva@yahoo.com
page: 47

MATERIALS AND METHODS

The study includes 12 patients with primary NSCLC who were admitted to the Clinic of Thoracic Surgery, St. Sofia University Hospital, Sofia, Bulgaria, between November 2007 and July 2008 and underwent a lobectomy for resection of tumor nodules. None had received prior therapy. Tissue acquisition was approved by the Institutional Ethics Committee and all participants signed informed consent forms. We analyzed 12 tumor samples and eight adjacent non cancerous lung tissues (as controls). All tumors were staged postoperatively according to the classification system of the International Union Against Cancer (UICC) and were early-stage NSCLCs. Five patients had squamous cell carcinoma (SCC) and seven patients had adenocarcinoma (AC). Clinical characteristics of the patients are summarized in Table 1. Total RNA was extracted from the tissue samples (RNeasy MiniKit; Qiagen, Hilden, Germany) and genomic DNA contamination was eliminated (RNAse-free DNAse set; Qiagen). RNA concentration was measured spectrophotometrically and the RNA integrity of all samples was tested by denaturing agarose electrophoresis. One μg of each RNA sample was used for cDNA synthesis (High Capacity Reverse Transcription Kit; Applied Biosystems, Foster City, CA, USA). Polymerase chain reaction (PCR) was performed on a 7500 Real Time PCR System (Applied Biosystems). Gene expression was analyzed using Human Apoptotic RT2 Profiler PCR Array (SuperArray Bioscience Corporation, Qiagen, Hilden, Germany). For expression analyses we used webbased software (available at www.sabiosciences. com), which automatically performs fold-change calculations based on the ΔΔCt method. The house-keeping gene RPL13A was used for data normalization. For each tumor, the fold change of each gene was calculated compared to the mean value of its expression in the control group. A gene was considered to be down or up regulated if its expression was altered more than 4-fold in at least half of the analyzed NSCLCs. For statistical evaluation of the data we used the Student’s t-test and the non parametrical tests of Shapiro-Wilk and Mann-Whitney. A p-value of 0.05 was accepted as the threshold for significant difference between the tumor and non tumor samples (Table 2).



Number 27
VOL. 27 (1), 2024
Number 26
Number 26 VOL. 26(2), 2023 All in one
Number 26
VOL. 26(2), 2023
Number 26
VOL. 26, 2023 Supplement
Number 26
VOL. 26(1), 2023
Number 25
VOL. 25(2), 2022
Number 25
VOL. 25 (1), 2022
Number 24
VOL. 24(2), 2021
Number 24
VOL. 24(1), 2021
Number 23
VOL. 23(2), 2020
Number 22
VOL. 22(2), 2019
Number 22
VOL. 22(1), 2019
Number 22
VOL. 22, 2019 Supplement
Number 21
VOL. 21(2), 2018
Number 21
VOL. 21 (1), 2018
Number 21
VOL. 21, 2018 Supplement
Number 20
VOL. 20 (2), 2017
Number 20
VOL. 20 (1), 2017
Number 19
VOL. 19 (2), 2016
Number 19
VOL. 19 (1), 2016
Number 18
VOL. 18 (2), 2015
Number 18
VOL. 18 (1), 2015
Number 17
VOL. 17 (2), 2014
Number 17
VOL. 17 (1), 2014
Number 16
VOL. 16 (2), 2013
Number 16
VOL. 16 (1), 2013
Number 15
VOL. 15 (2), 2012
Number 15
VOL. 15, 2012 Supplement
Number 15
Vol. 15 (1), 2012
Number 14
14 - Vol. 14 (2), 2011
Number 14
The 9th Balkan Congress of Medical Genetics
Number 14
14 - Vol. 14 (1), 2011
Number 13
Vol. 13 (2), 2010
Number 13
Vol.13 (1), 2010
Number 12
Vol.12 (2), 2009
Number 12
Vol.12 (1), 2009
Number 11
Vol.11 (2),2008
Number 11
Vol.11 (1),2008
Number 10
Vol.10 (2), 2007
Number 10
10 (1),2007
Number 9
1&2, 2006
Number 9
3&4, 2006
Number 8
1&2, 2005
Number 8
3&4, 2004
Number 7
1&2, 2004
Number 6
3&4, 2003
Number 6
1&2, 2003
Number 5
3&4, 2002
Number 5
1&2, 2002
Number 4
Vol.3 (4), 2000
Number 4
Vol.2 (4), 1999
Number 4
Vol.1 (4), 1998
Number 4
3&4, 2001
Number 4
1&2, 2001
Number 3
Vol.3 (3), 2000
Number 3
Vol.2 (3), 1999
Number 3
Vol.1 (3), 1998
Number 2
Vol.3(2), 2000
Number 2
Vol.1 (2), 1998
Number 2
Vol.2 (2), 1999
Number 1
Vol.3 (1), 2000
Number 1
Vol.2 (1), 1999
Number 1
Vol.1 (1), 1998

 

 


 About the journal ::: Editorial ::: Subscription ::: Information for authors ::: Contact
 Copyright © Balkan Journal of Medical Genetics 2006