THE MULTICOLOR FLUORESCENCE IN SITU HYBRIDIZATION (mFISH) HOMEPAGE
Liehr T*
*Corresponding Author: Dr. Thomas Liehr, Institut für Humangenetik und Anthropologie, Kollegiengasse 10, D-07743 Jena, Germany; Tel.: +49-3641-935533; Fax: +49-3641-935582;E-mail: i8lith@mti.uni-jena.de
page: 27

INTRODUCTION

The discipline of cytogenetics went through many stages of development, each providing more possibility for characterization of normal and structurally abnormal chromosomes [1]. Characterization of such chromosomes in humans is of high clinical importance and is the requisite condition for further molecular investigation aimed at identi- fication of disease-related genes [2,3] or positional cloning strategies [4]. Also, cytogenetic studies are significant for understanding the evolution of different animal species including Hominoids [5]. In the last 25-30 years, studies were accomplished by molecular cytogenetics, i.e., fluorescence in situ hybridization (FISH). In 1969, Gall and Pardue [6], introduced in situ hybridization, which, for the first time, allowed nucleic acid sequences to be examined inside cells or on chromosomes. However, because radioactivity has well-known shortcomings [7], the radioactive probe for labeling was replaced by alternative labeling modalities or detection systems, e.g., biotin detected by binding to avidin coupled to a fluorochrome [8]. Pinkel and coworkers [9] described the FISH technique that could be applied to human chromosomes and very soon thereafter the first twocolor FISH study was published [10]. We have defined multicolor FISH (mFISH) as the simultaneous use of at least three different ligands or fluo rochromes for the specific labeling of DNA (excluding the counter stain) [11]. According to this definition, the first successful mFISH experiments were those of Nederlof and coworkers [12] who visualized three differently-labeled nucleic acid sequences, simultaneously in blue [amino methyl coumarin acetic acid (AMCA)], in red [tetramethylrhodamine isothiocyanate (TRITC)] and in green [fluo rescein isothiocyanate (FITC)].Multicolor FISH assays have become indispensable for precise descriptions of chromosomal rearrangements, and studies including radiobiology, mutagenesis, inter-phase-architecture and chromosomal evolution. As the literature on this topic is huge, we created a regularly updated internet site in which all studies that apply mFISH are collected.




Number 22
VOL. 22(1), 2019
Number 22
VOL. 22, 2019 Accepted articles
Number 22
VOL. 22, 2019 Supplement
Number 21
VOL. 21(2), 2018
Number 21
VOL. 21 (1), 2018
Number 21
VOL. 21, 2018 Accepted articles
Number 21
VOL. 21, 2018 Supplement
Number 20
VOL. 20 (2), 2017
Number 20
VOL. 20 (1), 2017
Number 19
VOL. 19 (2), 2016
Number 19
VOL. 19 (1), 2016
Number 18
VOL. 18 (2), 2015
Number 18
VOL. 18 (1), 2015
Number 17
VOL. 17 (2), 2014
Number 17
VOL. 17 (1), 2014
Number 16
VOL. 16 (2), 2013
Number 16
VOL. 16 (1), 2013
Number 15
VOL. 15 (2), 2012
Number 15
VOL. 15, 2012 Supplement
Number 15
Vol. 15 (1), 2012
Number 14
14 - Vol. 14 (2), 2011
Number 14
The 9th Balkan Congress of Medical Genetics
Number 14
14 - Vol. 14 (1), 2011
Number 13
Vol. 13 (2), 2010
Number 13
Vol.13 (1), 2010
Number 12
Vol.12 (2), 2009
Number 12
Vol.12 (1), 2009
Number 11
Vol.11 (2),2008
Number 11
Vol.11 (1),2008
Number 10
Vol.10 (2), 2007
Number 10
10 (1),2007
Number 9
1&2, 2006
Number 9
3&4, 2006
Number 8
1&2, 2005
Number 8
3&4, 2004
Number 7
1&2, 2004
Number 6
3&4, 2003
Number 6
1&2, 2003
Number 5
3&4, 2002
Number 5
1&2, 2002
Number 4
Vol.3 (4), 2000
Number 4
Vol.2 (4), 1999
Number 4
Vol.1 (4), 1998
Number 4
3&4, 2001
Number 4
1&2, 2001
Number 3
Vol.3 (3), 2000
Number 3
Vol.2 (3), 1999
Number 3
Vol.1 (3), 1998
Number 2
Vol.3(2), 2000
Number 2
Vol.1 (2), 1998
Number 2
Vol.2 (2), 1999
Number 1
Vol.3 (1), 2000
Number 1
Vol.2 (1), 1999
Number 1
Vol.1 (1), 1998

 

 


 About the journal ::: Editorial ::: Subscription ::: Information for authors ::: Contact
 Copyright © Balkan Journal of Medical Genetics 2006