Both extra embryonic tissue and amniotic fluid tissue cultures were set up in two separate tissue culture flasks (Greiner, Frickenhausen, Germany) with Amniomed culture medium (Biochrom, Berlin, Germany). On the 7th day of culture, the medium was changed, and when an appropriate number of mitoses was observed, the cells were harvested [8]. Chromosomes were Giemsa-trypsin (GTG) banded and at least 20 metaphases were analyzed from each sample.

Genetic counseling was given in two sessions to couples. During the first session, couples were informed about the suspicious genetic abnormality and genetic diagnosis methods after pedigree analysis. When karyotype analyses were completed and triploidy was detected, the parents were informed about the triploidy and the recurrence risks for individual karyotype findings during the second genetic counseling session. Detailed USG examination was performed in every pregnancy at 18 or 19 weeks of gestation. Pathological examination was performed in all abort ed or terminated fetuses.