
IN VITRO ANALYSIS OF AKR1D1 INTERACTIONS WITH CLOPIDOGREL: EFFECTS ON ENZYME ACTIVITY AND GENE EXPRESSION Shutevska K1*, Kadifkova Panovska T1, Zhivikj Z1, Kapedanovska Nestorovska A2 *Corresponding Author: *Corresponding Author: Kristina Shutevska, Majka Tereza 47, 1000 Skopje, Republic of North Macedonia, +389 2 1326032 (142), k.sutevska@ff.ukim.edu.mk page: 69
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REFERENCES
Roffi M, Patrono C, Collet JP, Mueller C, Valgimigli
M, Andreotti F, et al. 2015 ESC Guidelines for the
management of acute coronary syndromes in pa-
tients presenting without persistent ST-segment el-
evation: Task Force for the Management of Acute
Coronary Syndromes in Patients Presenting without
Persistent ST-Segment Elevation of the European
Society of Cardiology (ESC). Eur Heart J. 2016 Jan
14;37(3):267315.
2.
Geisler T, Schaeffeler E, Dippon J, Winter S, Buse V,
Bischofs C, et al. CYP2C19 and nongenetic factors
predict poor responsiveness to clopidogrel loading
dose after coronary stent implantation. Pharmacog-
enomics. 2008 Sep;9(9):12519.
3.
Qu J, Zhang H, Rao C, Chen S, Zhao Y, Sun H, et
al. Dual Antiplatelet Therapy with Clopidogrel and
Aspirin Versus Aspirin Monotherapy in Patients Un-
dergoing Coronary Artery Bypass Graft Surgery. J
Am Heart Assoc. 2021 Jun;10(11):e020413.
4.
Tan BEX, Wong PY, Baibhav B, Thakkar S, Azhar
AZ, Rao M, et al. Clopidogrel Vs Aspirin Mono-
therapy Following Dual Antiplatelet Therapy After
Percutaneous Coronary Intervention: A Systematic
Review and Meta-analysis. Current Problems in Car-
diology. 2023 Aug 1;48(8):101174.
5.
Gurbel PA, Tantry US. Clopidogrel resistance?
Thromb Res. 2007;120(3):31121.
6.
Hawken TN, Berenson AM, Klopfenstein J, Leithead
CC, Brinster CJ, Sternbergh WC, et al. Clopidogrel
Resistance Is Common in Patients Undergoing Vas-
cular and Coronary Interventions. Journal of Vascular
Surgery. 2021 Jan 1;73(1):e256.
7.
Ma TKW, Lam YY, Tan VP, Yan BP. Variability in
response to clopidogrel: how important are pharma-
cogenetics and drug interactions? British Journal of
Clinical Pharmacology. 2011 Oct;72(4):697.
8.
Ray S. Clopidogrel resistance: The way forward. In-
dian Heart Journal. 2014 Sep;66(5):530.
9.
Giantini A, Timan IS, Dharma R, Sukmawan R,
Setiabudy R, Alwi I, et al. The role of clopidogrel
resistance-related genetic and epigenetic factors in
major adverse cardiovascular events among patients
with acute coronary syndrome after percutaneous
coronary intervention. Frontiers in Cardiovascular
Medicine. 2023 Feb 8;9:1027892.
10. Sangkuhl K, Klein TE, Altman RB. Clopidogrel
pathway. Pharmacogenetics and Genomics. 2010
Jul;20(7):463.
11. Savi P, Herbert JM, Pflieger AM, Dol F, Delebassee D,
Combalbert J, et al. Importance of hepatic metabolism
in the antiaggregating activity of the thienopyridine
clopidogrel. Biochemical Pharmacology. 1992 Aug
4;44(3):52732.
12. Jiang XL, Samant S, Lesko LJ, Schmidt S. Clinical
pharmacokinetics and pharmacodynamics of clopi-
dogrel. Clin Pharmacokinet. 2015 Feb;54(2):14766.
13. Pan Y, Chen W, Xu Y, Yi X, Han Y, Yang Q, et al.
Genetic Polymorphisms and Clopidogrel Efficacy for
Acute Ischemic Stroke or Transient Ischemic Attack.
Circulation. 2017 Jan 3;135(1):2133.
14. Hou X, Shi J, Sun H. Gene polymorphism of cy-
tochrome P450 2C19*2 and clopidogrel resistance
reflected by platelet function assays: a meta-analysis.
Eur J Clin Pharmacol. 2014 Sep;70(9):10417.
15. Ford NF. The Metabolism of Clopidogrel: CY-
P2C19 Is a Minor Pathway. J Clin Pharmacol. 2016
Dec;56(12):147483.
16. Zanger UM, Schwab M. Cytochrome P450 en-
zymes in drug metabolism: Regulation of gene ex-
pression, enzyme activities, and impact of genetic
variation. Pharmacology & Therapeutics. 2013 Apr
1;138(1):10341.
17. Barski OA, Tipparaju SM, Bhatnagar A. The aldo-
keto reductase superfamily and its role in drug
metabolism and detoxification. Drug Metab Rev.
2008;40(4):553624.
18. Nikolaou N, Gathercole LL, Kirkwood L, Dunford JE,
Hughes BA, Gilligan LC, et al. AKR1D1 regulates
glucocorticoid availability and glucocorticoid recep-
tor activation in human hepatoma cells. The Journal
of Steroid Biochemistry and Molecular Biology. 2019
May 1;189:21827.
19. Penning TM, Wangtrakuldee P, Auchus RJ. Struc-
tural and Functional Biology of Aldo-Keto Reductase
Steroid-Transforming Enzymes. Endocr Rev. 2019
Apr 1;40(2):44775.
20. Chen M, Penning TM. 5β-Reduced steroids and hu-
man Δ(4)-3-ketosteroid 5β-reductase (AKR1D1).
Steroids. 2014 May;83:1726.
21. Chaudhry AS, Thirumaran RK, Yasuda K, Yang X,
Fan Y, Strom SC, et al. Genetic Variation in Aldo-
Keto Reductase 1D1 ( AKR1D1 ) Affects the Expres-
sion and Activity of Multiple Cytochrome P450s.
Drug Metab Dispos. 2013 Aug;41(8):153847.
22. Kapedanovska-Nestorovska A, Dimovski AJ, Sterjev
Z, Matevska Geskovska N, Suturkova L, Ugurov P,
et al. The AKR1D1*36 (rs1872930) Allelic Variant
Is Independently Associated With Clopidogrel Treat-
ment Outcome. Pharmgenomics Pers Med . 2019 Oct;
12:28795.
23. Green MR, Sambrook J. Molecular Cloning: A Labo-
ratory Manual. 4th ed. Cold Spring Harbor (NY):
Cold Spring Harbor Laboratory Press; 2012..
24. Brown TA. Gene Cloning and DNA Analysis: An In-
troduction. 8th ed. Hoboken (NJ): Wiley-Blackwell;
2020..
25. Shutevska K, Nestorovska AK. Cloning, expression,
and purification of recombinant AKR1D1 for thera-
peutic applications. Acta Pharm. 2024 Dec 17; doi:
10.2478/acph-2025-0003. Online ahead of print.
26. Möller G, Temml V, Cala Peralta A, Gruet O, Rich-
omme P, Séraphin D, et al. Analogues of Natural
Chalcones as Efficient Inhibitors of AKR1C3. Me-
tabolites. 2022 Feb;12(2):99.
27. Nikolaou N, Gathercole LL, Marchand L, Althari S,
Dempster NJ, Green CJ, et al. AKR1D1 is a novel
regulator of metabolic phenotype in human hepato-
cytes and is dysregulated in non-alcoholic fatty liver
disease. Metabolism. 2019 Oct;99:6780.
28. Kędzierski J, Allard JA, Odermatt A, Smieško M.
Assessment of the inhibitory potential of anabolic
steroids towards human AKR1D1 by computational
methods and in vitro evaluation. Toxicology Letters.
2023 Aug 1;384:113.
29. Laurence L. Brunton, Randa Hilal-Dandan, Bjorn
Knollmann, editors. Goodman & Gilmans The Phar-
macological Basis of Therapeutics. 13th ed. New
York, NY, USA: McGraw Hill Education, 2017.
30. Rižner TL, Penning TM. Role of Aldo-Keto Reduc-
tase Family 1 (AKR1) Enzymes in Human Steroid
Metabolism. Steroids. 2013 Nov 1;79:10.1016/j.ster-
oids.2013.10.012.
31. Chen M, Drury JE, Penning TM. Substrate specificity
and inhibitor analyses of human steroid 5β-reductase
(AKR1D1). Steroids. 2011 Apr;76(5):48490.
32. Chen M, Drury JE, Christianson DW, Penning
TM. Conversion of Human Steroid 5β-Reductase
(AKR1D1) into 3β-Hydroxysteroid Dehydrogenase
by Single Point Mutation E120H. Journal of Biologi-
cal Chemistry. 2012 May 11;287(20):1660922.
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