ASSOCIATION OF 308TNF AND +252LTA SINGLE NUCLEOTIDE POLYMORPHISMS WITH HEMATOLOGICAL MALIGNANCIES IN CHILDREN FROM THE BASHKORTOSTAN REPUBLIC
Yakupova EV 1,* Krasavtceva TN2, Malyevsky OA2, Viktorova TV1
*Corresponding Author: Dr. Elvira V. Yakupova, Institute of Biochemistry and Genetics, Ufa Research Centre, Russian Academy of Science, Prospect Oktyabrya 69, Ufa 450054, Russia; Tel./Fax: +007-3472-356088; E-mail: ecolab_203@mail.ru
page: 9

RESULTS

Distributions of genotype and allele frequencies of the308GA TNF gene polymorphism are shown in Ta­ble 1. We have found a slightly increased TNF*GG geno­type frequency among hematological malignancy patients, but differences among analyzed groups were not signifi­cant (c2  = 0.38, p = 0.54). When the hematological ma­lignancies group were divided according to diagnosis, patients with ALL showed the same distribution of geno­types as the control group. We noted an increased fre­quency of the TNF*AA genotype among AML patients. The TNF*GG genotype frequency in the group of patient with NHL was the highest among the analyzed groups (c2   = 8.15, p = 0.005). The lowest frequency of the TNF*A allele was among NHL patients.

The distribution of genotype and allele frequencies of the +252AG LTA gene polymorphism among the hema­tological malignancy patients were similar to those ob­served in healthy controls (Table 2). But we did note some increase of the LTA*GG genotype among hematological malignancy patients (c2  = 1.54, p = .22). The LTA*AG genotype frequency was highest in the AML group of patients, and among patients with NHL, the LTA*AA genotype frequency was significantly higher (c2  = 9.12, p = 0.003).

The genotype combinations of the TNF/LTA genes are shown in Table 3. It is important to note that the frequency of the TNF/LTA genotype combinations with at least one mutant allele among AML patients was found in 80% of cases, more often than in any other group. Combinations with one or two mutant alleles in the NHL group were found in only 22.22%, furthermore, we have not found genotype combinations that included more than two mu­tant alleles in the NHL group.

 

Table 1. Genotype and allele frequency distributions (%) of the 308GA polymorphism of the promoter region of the TNF gene.

 

 

 

 

n

 

TNF*GG

 

TNF*GA

 

TNF*AA

 

TNF*G

 

TNF*A

 

Hematological malignancy patients

 

105

 

72.38

 

23.81

 

3.81

 

84.29

 

15.71

 

ALL patients

 

86

 

72.09

 

24.42

 

3.49

 

84.30

 

15.70

 

AML patients

 

10

 

60.00

 

30.00

 

10.00

 

75.00

 

25.00

 

NHL patients

 

9

 

88.89

 

11.11

 

00.00

 

94.44

 

5.56

 

Control subjects

 

131

 

67.18

 

31.30

 

1.52

 

82.38

 

17.17

 

Table 2. Genotype and allele frequency distributions (%) of the +252AG polymorphism of the LTA gene.

 

 

 

 

n

 

LTA*AA

 

LTA*AG

 

LTA*GG

 

LTA*A

 

LTA*G

 

Hematological malignancy patients

 

105

 

55.24

 

37.14

 

7.62

 

73.81

 

26.19

 

ALL patients

 

86

 

56.98

 

33.72

 

9.30

 

73.84

 

26.16

 

AML patients

 

10

 

20.00

 

80.00

 

00.00

 

60.00

 

40.00

 

NHL patients

 

9

 

77.78

 

22.22

 

00.00

 

88.89

 

11.11

 

Control subjects

 

141

 

54.61

 

42.55

 

2.84

 

75.89

 

24.11

 

Table 3 Association between 308TNF and +252LTA genotypes (%).

 

 

TNF/LTA

 

n

 

GG/AA

 

GG/AG

 

GG/GG

 

GA/AA

 

GA/AG

 

GA/GG

 

AA/AG

 

AA/GG

 

Hematological

malignancy

 

105

 

49.53

 

20.00

 

2.86

 

5.71

 

15.24

 

2.86

 

1.90

 

1.90

 

ALL

 

86

 

50.00

 

18.60

 

3.49

 

6.98

 

13.95

 

3.49

 

1.16

 

2.33

 

AML

 

10

 

20.00

 

40.00

 

00.00

 

00.00

 

30.00

 

00.00

 

10.00

 

00.00

 

NHL

 

9

 

77.78

 

11.11

 

00.00

 

00.00

 

11.11

 

00.00

 

00.00

 

00.00

 

Control subjects

 

126

 

47.62

 

20.63

 

0.79

 

6.35

 

21.44

 

1.59

 

0.79

 

0.79




Number 25
VOL. 25 (1), 2022
Number 24
VOL. 24(2), 2021
Number 24
VOL. 24(1), 2021
Number 23
VOL. 23(2), 2020
Number 22
VOL. 22(2), 2019
Number 22
VOL. 22(1), 2019
Number 22
VOL. 22, 2019 Supplement
Number 21
VOL. 21(2), 2018
Number 21
VOL. 21 (1), 2018
Number 21
VOL. 21, 2018 Supplement
Number 20
VOL. 20 (2), 2017
Number 20
VOL. 20 (1), 2017
Number 19
VOL. 19 (2), 2016
Number 19
VOL. 19 (1), 2016
Number 18
VOL. 18 (2), 2015
Number 18
VOL. 18 (1), 2015
Number 17
VOL. 17 (2), 2014
Number 17
VOL. 17 (1), 2014
Number 16
VOL. 16 (2), 2013
Number 16
VOL. 16 (1), 2013
Number 15
VOL. 15 (2), 2012
Number 15
VOL. 15, 2012 Supplement
Number 15
Vol. 15 (1), 2012
Number 14
14 - Vol. 14 (2), 2011
Number 14
The 9th Balkan Congress of Medical Genetics
Number 14
14 - Vol. 14 (1), 2011
Number 13
Vol. 13 (2), 2010
Number 13
Vol.13 (1), 2010
Number 12
Vol.12 (2), 2009
Number 12
Vol.12 (1), 2009
Number 11
Vol.11 (2),2008
Number 11
Vol.11 (1),2008
Number 10
Vol.10 (2), 2007
Number 10
10 (1),2007
Number 9
1&2, 2006
Number 9
3&4, 2006
Number 8
1&2, 2005
Number 8
3&4, 2004
Number 7
1&2, 2004
Number 6
3&4, 2003
Number 6
1&2, 2003
Number 5
3&4, 2002
Number 5
1&2, 2002
Number 4
Vol.3 (4), 2000
Number 4
Vol.2 (4), 1999
Number 4
Vol.1 (4), 1998
Number 4
3&4, 2001
Number 4
1&2, 2001
Number 3
Vol.3 (3), 2000
Number 3
Vol.2 (3), 1999
Number 3
Vol.1 (3), 1998
Number 2
Vol.3(2), 2000
Number 2
Vol.1 (2), 1998
Number 2
Vol.2 (2), 1999
Number 1
Vol.3 (1), 2000
Number 1
Vol.2 (1), 1999
Number 1
Vol.1 (1), 1998

 

 


 About the journal ::: Editorial ::: Subscription ::: Information for authors ::: Contact
 Copyright © Balkan Journal of Medical Genetics 2006