ASSOCIATION OF –308TNF AND +252LTA SINGLE NUCLEOTIDE POLYMORPHISMS WITH HEMATOLOGICAL MALIGNANCIES IN CHILDREN FROM THE BASHKORTOSTAN REPUBLIC
Yakupova EV 1,* Krasavtceva TN2, Malyevsky OA2, Viktorova TV1
*Corresponding Author: Dr. Elvira V. Yakupova, Institute of Biochemistry and Genetics, Ufa Research Centre, Russian Academy of Science, Prospect Oktyabrya 69, Ufa 450054, Russia; Tel./Fax: +007-3472-356088; E-mail: ecolab_203@mail.ru
page: 9

RESULTS

Distributions of genotype and allele frequencies of the–308G®A TNF gene polymorphism are shown in Ta­ble 1. We have found a slightly increased TNF*GG geno­type frequency among hematological malignancy patients, but differences among analyzed groups were not signifi­cant (c2  = 0.38, p = 0.54). When the hematological ma­lignancies group were divided according to diagnosis, patients with ALL showed the same distribution of geno­types as the control group. We noted an increased fre­quency of the TNF*AA genotype among AML patients. The TNF*GG genotype frequency in the group of patient with NHL was the highest among the analyzed groups (c2   = 8.15, p = 0.005). The lowest frequency of the TNF*A allele was among NHL patients.

The distribution of genotype and allele frequencies of the +252A®G LTA gene polymorphism among the hema­tological malignancy patients were similar to those ob­served in healthy controls (Table 2). But we did note some increase of the LTA*GG genotype among hematological malignancy patients (c2  = 1.54, p = .22). The LTA*AG genotype frequency was highest in the AML group of patients, and among patients with NHL, the LTA*AA genotype frequency was significantly higher (c2  = 9.12, p = 0.003).

The genotype combinations of the TNF/LTA genes are shown in Table 3. It is important to note that the frequency of the TNF/LTA genotype combinations with at least one mutant allele among AML patients was found in 80% of cases, more often than in any other group. Combinations with one or two mutant alleles in the NHL group were found in only 22.22%, furthermore, we have not found genotype combinations that included more than two mu­tant alleles in the NHL group.

 

Table 1. Genotype and allele frequency distributions (%) of the –308G®A polymorphism of the promoter region of the TNF gene.

 

 

 

 

n

 

TNF*GG

 

TNF*GA

 

TNF*AA

 

TNF*G

 

TNF*A

 

Hematological malignancy patients

 

105

 

72.38

 

23.81

 

3.81

 

84.29

 

15.71

 

ALL patients

 

86

 

72.09

 

24.42

 

3.49

 

84.30

 

15.70

 

AML patients

 

10

 

60.00

 

30.00

 

10.00

 

75.00

 

25.00

 

NHL patients

 

9

 

88.89

 

11.11

 

00.00

 

94.44

 

5.56

 

Control subjects

 

131

 

67.18

 

31.30

 

1.52

 

82.38

 

17.17

 

Table 2. Genotype and allele frequency distributions (%) of the +252A®G polymorphism of the LTA gene.

 

 

 

 

n

 

LTA*AA

 

LTA*AG

 

LTA*GG

 

LTA*A

 

LTA*G

 

Hematological malignancy patients

 

105

 

55.24

 

37.14

 

7.62

 

73.81

 

26.19

 

ALL patients

 

86

 

56.98

 

33.72

 

9.30

 

73.84

 

26.16

 

AML patients

 

10

 

20.00

 

80.00

 

00.00

 

60.00

 

40.00

 

NHL patients

 

9

 

77.78

 

22.22

 

00.00

 

88.89

 

11.11

 

Control subjects

 

141

 

54.61

 

42.55

 

2.84

 

75.89

 

24.11

 

Table 3 Association between –308TNF and +252LTA genotypes (%).

 

 

TNF/LTA

 

n

 

GG/AA

 

GG/AG

 

GG/GG

 

GA/AA

 

GA/AG

 

GA/GG

 

AA/AG

 

AA/GG

 

Hematological

malignancy

 

105

 

49.53

 

20.00

 

2.86

 

5.71

 

15.24

 

2.86

 

1.90

 

1.90

 

ALL

 

86

 

50.00

 

18.60

 

3.49

 

6.98

 

13.95

 

3.49

 

1.16

 

2.33

 

AML

 

10

 

20.00

 

40.00

 

00.00

 

00.00

 

30.00

 

00.00

 

10.00

 

00.00

 

NHL

 

9

 

77.78

 

11.11

 

00.00

 

00.00

 

11.11

 

00.00

 

00.00

 

00.00

 

Control subjects

 

126

 

47.62

 

20.63

 

0.79

 

6.35

 

21.44

 

1.59

 

0.79

 

0.79




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