Balkan Journal of Medical Genetics

RESULTS OF LIQUID BIOPSY STUDIES BY NEXT GENERATION SEQUENCING IN PATIENTS WITH ADVANCED STAGE NON-SMALL CELL LUNG CANCER: SINGLE CENTER EXPERIENCE FROM TURKEY
Buyuksimsek M1,*, Togun M2, Oguz Kara I1, Bisgin A3,4, Boga I4, Tohumcuoglu M1, Ogul A1, Evren Yetisir A1, Sahin B1, Erdem Sumbul H5, Mirili C6
*Corresponding Author: Mahmut Buyuksimsek, M.D., Department of Oncology, Çukurova University Faculty of Medicine, Sarican, Adana, Turkey. Tel: +90-536-862-20-26. Fax: +90-322-338-70-72. E-mail: mahmutbuyuksimsek@gmail.com
page: 17

INTRODUCTION

Non-small cell lung cancer (NSCLC) is an important cause of morbidity and mortality worldwide. Many patients with NSCLS are diagnosed at advanced stages and treated with targeted therapy and immunotherapy in addition to systemic chemotherapy [1]. The College of American Pathologists (CAP), International Association for the Study of Lung Cancer (IASLC) and Association for Molecular Pathology (AMP) recommended that EGFR, ALK, and ROS1 were necessary tests in advanced stage NSCLC patients whose tumors contain an element of adenocarcinoma (AC) in their 2018 updated testing guideline. Moreover, the results of the recent clinical data indicate those panels including BRAF, MET, RET, ERBB2 and KRAS, should be used at a minimum [2]. Cell-free circulating tumor DNA (ccfDNA), which is released from the tumor into the systemic circulation, is used in the liquid biopsy [3]. Thompson et al. [4] and Schwaederlé et al. [5] demonstrated that next generation sequencing (NGS) of plasmabased ccfDNA can be used to assess mutations in NSCLC. Several recent retrospective and prospective studies also used plasma samples to decide targeted treatments [6,7]. The NGS used for the analysis of ccfDNA involves reading of the DNA strand 10,000-times using deep sequencing and allows determination of the type and frequency of a given mutation by bioinformatic analyses. It is possible to identify single base mutations, short insertions and deletions, wide genomic deletions, or rearrangements such as inversion and translocation (by amplifications) using NGS [8]. In the present study, we aimed to present our NGS results of liquid biopsy samples that is increasingly used in clinical practice for NSCLC that comprises several genetic alterations guiding therapeutic opportunities.

Number 27 VOL. 27 (2), 2024	Number 27 VOL. 27 (1), 2024
Number 26 Number 26 VOL. 26(2), 2023 All in one	Number 26 VOL. 26(2), 2023
Number 26 VOL. 26, 2023 Supplement	Number 26 VOL. 26(1), 2023
Number 25 VOL. 25(2), 2022	Number 25 VOL. 25 (1), 2022
Number 24 VOL. 24(2), 2021	Number 24 VOL. 24(1), 2021
Number 23 VOL. 23(2), 2020	Number 22 VOL. 22(2), 2019
Number 22 VOL. 22(1), 2019	Number 22 VOL. 22, 2019 Supplement
Number 21 VOL. 21(2), 2018	Number 21 VOL. 21 (1), 2018
Number 21 VOL. 21, 2018 Supplement	Number 20 VOL. 20 (2), 2017
Number 20 VOL. 20 (1), 2017	Number 19 VOL. 19 (2), 2016
Number 19 VOL. 19 (1), 2016	Number 18 VOL. 18 (2), 2015
Number 18 VOL. 18 (1), 2015	Number 17 VOL. 17 (2), 2014
Number 17 VOL. 17 (1), 2014	Number 16 VOL. 16 (2), 2013
Number 16 VOL. 16 (1), 2013	Number 15 VOL. 15 (2), 2012
Number 15 VOL. 15, 2012 Supplement	Number 15 Vol. 15 (1), 2012
Number 14 14 - Vol. 14 (2), 2011	Number 14 The 9th Balkan Congress of Medical Genetics
Number 14 14 - Vol. 14 (1), 2011	Number 13 Vol. 13 (2), 2010
Number 13 Vol.13 (1), 2010	Number 12 Vol.12 (2), 2009
Number 12 Vol.12 (1), 2009	Number 11 Vol.11 (2),2008
Number 11 Vol.11 (1),2008	Number 10 Vol.10 (2), 2007
Number 10 10 (1),2007	Number 9 1&2, 2006
Number 9 3&4, 2006	Number 8 1&2, 2005
Number 8 3&4, 2004	Number 7 1&2, 2004
Number 6 3&4, 2003	Number 6 1&2, 2003
Number 5 3&4, 2002	Number 5 1&2, 2002
Number 4 Vol.3 (4), 2000	Number 4 Vol.2 (4), 1999
Number 4 Vol.1 (4), 1998	Number 4 3&4, 2001
Number 4 1&2, 2001	Number 3 Vol.3 (3), 2000
Number 3 Vol.2 (3), 1999	Number 3 Vol.1 (3), 1998
Number 2 Vol.3(2), 2000	Number 2 Vol.1 (2), 1998
Number 2 Vol.2 (2), 1999	Number 1 Vol.3 (1), 2000
Number 1 Vol.2 (1), 1999	Number 1 Vol.1 (1), 1998

About the journal ::: Editorial ::: Subscription ::: Information for authors ::: Contact