Balkan Journal of Medical Genetics

MicroRNAs IN BREAST CANCER —OUR INITIAL RESULTS
Popovska-Jankovic K1, Noveski P1, Chakalova L1, Petrusevska G2, Kubelka K3, Plaseska-Karanfilska D1
*Corresponding Author: Professor Dr. Dijana Plaseska-Karanfilska, Research Centre for Genetic Engineering and Biotechnology “Georgi D. Efremov,” Macedonian Academy of Sciences and Arts, Krste Misirkov 2, Skopje 1000, Republic of Macedonia; Tel: +389(0)2 3235410; Fax: +389 (0)2 3115434; E-mail: dijana@manu.edu.mk
page: 87

RESULTS AND DISCUSSION

Here we present our initial results of miRNA analysis of breast cancer and normal tissues that included quantitative ReTi-PCR (qPCR) involving stemloop RT primers combined with TaqMan® PCR (Life Technologies) and miRNA microarray analyses. A total of 35 patients with breast cancer were analyzed using ReTi-PCR of four miRNAs (miR-155, miR-21, miR-125b and miR-145). Our initial results showed that miR-155 and miR-21 are up regulated and miR- 125b and miR-145 are down regulated in breast cancer. Our results are in agreement with other published studies for miRNA expression profiling in breast cancer [7,8,11,12,15,16]. MicroRNA microarray analysis was performed in several patients and the data were analyzed by R and MeV statistical programs. Nine miRNAs were differentialy expressed, of which seven were up regulated (miR-155, miR-146a, miR-150, miR-210, miR-21, miR-106b and miR-142-3p) and two were down regulated (miR-139-5p and miR-320c). These up regulated miRNAs were also shown to be differentially expressed in breast cancer by other authors [7- 9,15]. Nevertheless, additional testing and analyses are needed to establish accurate and precise miRNA markers that will contribute to the improvement of diagnosis and prognosis in breast cancer patients.

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