SIMULTANEOUS DETECTION OF FACTOR V LEIDEN AND FACTOR II G20210A VARIANTS BY A MULTIPLEX PCR ON WHOLE BLOOD
Djordjevic V, Rakicevic L, Gagic M, Nikolic A, Savic A
*Corresponding Author: Valentina Djordjevic, Laboratory for Molecular Biology, Institute of Molecular Genetics and Genetic Engineering, Vojvode Stepe 444a, P.O. Box 446, 11001 Belgrade, Yugoslavia; Tel: +381 11 3976658; Fax +381 11 3975 808; E-mail qwert@eunet.yu
page: 15

Abstract

Diagnosis of factor V G1691A (factor V Leiden) and of factor II G20210A mutations is important for patients with thrombophilia or thrombophilic conditions. A variety of methods based on polymerase chain reaction amplification of the corresponding gene fragments have been described to detect these mutations. We have developed a method based on polymerase chain reaction for simultaneous detection of factor V Leiden and factor II G20210A from whole blood, without modification of the primers previously described for separate detection of these mutations. This method is equally informative, more rapid and less expensive, than individual detection of these mutations.

Key words: factor V Leiden, factor II G20210A, multiplex PCR.




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