THE ANGIOTENSIN-CONVERTING ENZYME GENE POLYMORPHISM IS ASSOCIATED WITH PREGNANCY MISCARRIAGE AND PLACENTAL INSUFFICIENCY
Bespalova ON, Ivashchenko TE, Tarasenko OA, Demin GS, Ajlamazjan EK, Baranov VS*
*Corresponding Author: Professor Vladislav S. Baranov, Laboratory for Prenatal Diagnostics, Scientific Research Institute of Obstetrics and Gynecology D.O. Otta, Russian Academy of Medical Science, Mendeleev­skaya line 3, 199034 Saint Petersburg, Russia; Tel.: +7-812-328-0487; Fax: +7-812-328-0487; E-mail: Baranov@vb2475.spb.edu
page: 3

MATERIALS AND METHODS

Samples of placentae were obtained from 137 parturient women from the Maternity Department at the Institute of Obstetrics and Gynecology D.O. Otta of the Russian Academy of Medical Science, Saint Petersburg, Russia. Approval for this study was granted by the local Human Institutional Investigation Committee. They were classified into four groups as follows: group 1 (controls) was composed of 36 parturient women negative for both PM and PI in the present pregnancy (supported by clinical-laboratory, ultrasonic, Doppler and morphological methods), group 2 comprised 54 parturient women negative for PM in their obstetric history but positive for PI in the current pregnancy, group 3 composed 23 parturient women positive for PM (with one or more spontaneous abortions, and/or premature birth in their obstetric history) but negative for PI in the present pregnancy, and group 4 comprised 24 parturient women with PM in their obstetric history and positive for PI in the current pregnancy.

DNA extraction from placental tissue was carried out according to the technique by Sambrook et al. [16] with some modifications. The I/D polymorphism of the ACE gene was determined by a polymerase chain reaction (PCR) method. Amplification was carried out in a total volume 25 mL that contained 15 nM of each primer (ACE F: 5'-CTG GAG ACC ACT CCC ATC CTT TCT-3', ACE R: 5'-GAT GTG GCC ATC ACA TTC GTC AGA T-3'), 67 mM Tris-Hcl (pH 8.8), 16.6 mM of ammonium sulfate, 6.7 mM MgCl2, 6.7 mM EDTA, 10 mM mercaptoethanol, 170 mkg BSA, 1.0 mM of each dNTP and one1 of Taq-polymerase (‘Bion’, Moscow, Russia). The PCR was performed under the following conditions: denaturation at 94°C for 7 min., followed by 30 cycles of amplification at 94°C for 40 seconds, 60°C for 40 seconds, 72°C for 1 min. and 72°C for 5 min. The DNA fragments were separated in a 7% polyacrylamide gel and subsequently stained with ethidium bromide and visualized under ultraviolet light.

A standard chi-square test was used for comparing the genotype and allele frequencies. The relative risk was estimated by odds ratio (OR) and p values of <0.05 were considered to be significant. All confidence intervals (CIs) were calculated at the 95% level. Statistical analysis of results was performed using ‘STATISTICA v5.5a’ software.




Number 23
VOL. 23, 2020 Acepted articles
Number 22
VOL. 22(2), 2019
Number 22
VOL. 22(1), 2019
Number 22
VOL. 22, 2019 Accepted articles
Number 22
VOL. 22, 2019 Supplement
Number 21
VOL. 21(2), 2018
Number 21
VOL. 21 (1), 2018
Number 21
VOL. 21, 2018 Accepted articles
Number 21
VOL. 21, 2018 Supplement
Number 20
VOL. 20 (2), 2017
Number 20
VOL. 20 (1), 2017
Number 19
VOL. 19 (2), 2016
Number 19
VOL. 19 (1), 2016
Number 18
VOL. 18 (2), 2015
Number 18
VOL. 18 (1), 2015
Number 17
VOL. 17 (2), 2014
Number 17
VOL. 17 (1), 2014
Number 16
VOL. 16 (2), 2013
Number 16
VOL. 16 (1), 2013
Number 15
VOL. 15 (2), 2012
Number 15
VOL. 15, 2012 Supplement
Number 15
Vol. 15 (1), 2012
Number 14
14 - Vol. 14 (2), 2011
Number 14
The 9th Balkan Congress of Medical Genetics
Number 14
14 - Vol. 14 (1), 2011
Number 13
Vol. 13 (2), 2010
Number 13
Vol.13 (1), 2010
Number 12
Vol.12 (2), 2009
Number 12
Vol.12 (1), 2009
Number 11
Vol.11 (2),2008
Number 11
Vol.11 (1),2008
Number 10
Vol.10 (2), 2007
Number 10
10 (1),2007
Number 9
1&2, 2006
Number 9
3&4, 2006
Number 8
1&2, 2005
Number 8
3&4, 2004
Number 7
1&2, 2004
Number 6
3&4, 2003
Number 6
1&2, 2003
Number 5
3&4, 2002
Number 5
1&2, 2002
Number 4
Vol.3 (4), 2000
Number 4
Vol.2 (4), 1999
Number 4
Vol.1 (4), 1998
Number 4
3&4, 2001
Number 4
1&2, 2001
Number 3
Vol.3 (3), 2000
Number 3
Vol.2 (3), 1999
Number 3
Vol.1 (3), 1998
Number 2
Vol.3(2), 2000
Number 2
Vol.1 (2), 1998
Number 2
Vol.2 (2), 1999
Number 1
Vol.3 (1), 2000
Number 1
Vol.2 (1), 1999
Number 1
Vol.1 (1), 1998

 

 


 About the journal ::: Editorial ::: Subscription ::: Information for authors ::: Contact
 Copyright © Balkan Journal of Medical Genetics 2006