THE +3953 (C->T) POLYMORPHISM OF INTERLEUKIN-1β GENE IN MYOCARDIAL INFARCTION IN TATARS AND RUSSIANS FROM BASHKORTOSTAN
Tulyakova G1,*, Nasibullin T1, Zakirova A2, Khusnutdinova E1, Mustafina O1
*Corresponding Author: Dr. Gulnara Tulyakova, Institute of Biochemistry and Genetics, Ufa Research Center, Russian Academy of Sciences, October Avenue 69, 450054 Ufa, Bashkortostan, Russia; Tel.: +7-3472-361176; Fax: +7-3472-356100; E-mail: gulnarat@mail.ru
page: 65

MATERIALS AND METHODS

We studied DNA samples from 306 male patients with MI (191 Russians aged 45.5  0.5 years, and 115 Tatars aged 45.03  0.6), attending the Republic Cardiac Center and Republic Clinical Hospital in Ufa. The MI was diagnosed on the basis of clinical, enzymatic, electrocardio­graphic and echocardiographic criteria. All of the following criteria were necessary to confirm this diagnosis: typical chest pain for longer than 30 minutes, an increase in creatine kinase of more than twice the baseline level, characteristic electrocardiographic changes with Q-wave and zone of hypokinesiasia or akinesia. The control group consisted of 245 unrelated healthy male blood donors (116 Russians aged 38.6  0.8, and 129 Tatars aged 39.7  0.7).

      Genomic DNA was extracted from whole venous blood by phenol-chloroform extraction [10]. Genotyping was performed by polymerase chain reaction (PCR). The primers 5'-GTT GTC ATC AGA CTT TGA CC-3' and 5'-TTC AGT TCA TAT GGA CCA GA-3' were used to amplify a DNA fragment of 249 bp containing the polymorphic TaqI site [11]. The PCR procedure consisted of three cycles of denaturation at 97C for 90 seconds, 55C for 90 seconds and 74C for 60 seconds, 32 cycles of denaturation at 97C for 30 seconds, annealing at 55C for 30 seconds, extension at 74C for 30 seconds, and a final extension at 72C for 10 min. [11]. This polymorphism is characterized by a T→C transition. The allele containing C at position +3953 and digested by TaqI restrictase is designated as allele E1. Another allele that was not digested by TaqI (T at position +3953), was named allele E2. The PCR products were analyzed after electrophoresis on a polyacrylamide gel stained with ethi­dium bromide. The E1 allele gave fragments of 135 and 114 bp, and the E2 allele gave a 249 bp fragment.

      Genotype frequencies were checked for deviation from the Hardy-Weinberg equilibrium by the λ2 test, using the Rows x Columns program [12]. The confidence interval (CI) of genotype frequencies were obtained on the basis of Fishers distribution. Pair-wise comparison of genotypes and alleles was done with Fishers two-side exact test [13]. The strength of association between the alleles and genotypes was estimated by the odds ratio (OR) [14]. Differences were considered significant at a value of p<0.05.




Number 26
VOL. 26(1), 2023
Number 25
VOL. 25(2), 2022
Number 25
VOL. 25 (1), 2022
Number 24
VOL. 24(2), 2021
Number 24
VOL. 24(1), 2021
Number 23
VOL. 23(2), 2020
Number 22
VOL. 22(2), 2019
Number 22
VOL. 22(1), 2019
Number 22
VOL. 22, 2019 Supplement
Number 21
VOL. 21(2), 2018
Number 21
VOL. 21 (1), 2018
Number 21
VOL. 21, 2018 Supplement
Number 20
VOL. 20 (2), 2017
Number 20
VOL. 20 (1), 2017
Number 19
VOL. 19 (2), 2016
Number 19
VOL. 19 (1), 2016
Number 18
VOL. 18 (2), 2015
Number 18
VOL. 18 (1), 2015
Number 17
VOL. 17 (2), 2014
Number 17
VOL. 17 (1), 2014
Number 16
VOL. 16 (2), 2013
Number 16
VOL. 16 (1), 2013
Number 15
VOL. 15 (2), 2012
Number 15
VOL. 15, 2012 Supplement
Number 15
Vol. 15 (1), 2012
Number 14
14 - Vol. 14 (2), 2011
Number 14
The 9th Balkan Congress of Medical Genetics
Number 14
14 - Vol. 14 (1), 2011
Number 13
Vol. 13 (2), 2010
Number 13
Vol.13 (1), 2010
Number 12
Vol.12 (2), 2009
Number 12
Vol.12 (1), 2009
Number 11
Vol.11 (2),2008
Number 11
Vol.11 (1),2008
Number 10
Vol.10 (2), 2007
Number 10
10 (1),2007
Number 9
1&2, 2006
Number 9
3&4, 2006
Number 8
1&2, 2005
Number 8
3&4, 2004
Number 7
1&2, 2004
Number 6
3&4, 2003
Number 6
1&2, 2003
Number 5
3&4, 2002
Number 5
1&2, 2002
Number 4
Vol.3 (4), 2000
Number 4
Vol.2 (4), 1999
Number 4
Vol.1 (4), 1998
Number 4
3&4, 2001
Number 4
1&2, 2001
Number 3
Vol.3 (3), 2000
Number 3
Vol.2 (3), 1999
Number 3
Vol.1 (3), 1998
Number 2
Vol.3(2), 2000
Number 2
Vol.1 (2), 1998
Number 2
Vol.2 (2), 1999
Number 1
Vol.3 (1), 2000
Number 1
Vol.2 (1), 1999
Number 1
Vol.1 (1), 1998

 

 


 About the journal ::: Editorial ::: Subscription ::: Information for authors ::: Contact
 Copyright © Balkan Journal of Medical Genetics 2006