INCREASED EXPRESSION OF PENTRAXIN 3 IN
PLACENTAL TISSUES FROM PATIENTS WITH
UNEXPLAINED RECURRENT PREGNANCY LOSS
Zeybek S1,*, Tepeli E2, Cetin GO3, Caner V3, Senol H4, Yildirim B2, Bagci G3
*Corresponding Author: Selcan Zeybek, M.D., Department of Medical Genetics, Erzurum Regional
Training and Research Hospital, Cat Volu Street, 25070, Erzurum, Turkey. Tel.: +90-506-399-2644.
Fax: +90-442-232-5025. E-mail: selcankesan@yahoo.com
page: 21
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RESULTS
The characteristics of the URPL patients are shown
in Table 2. Patient and control groups did not differ significantly
in terms of age (p = 0.819), and 30 (60.0%), 17
(34.0%), and three (6.0%) URPL patients had zero, one,
and two live births, respectively. Numbers of previous
miscarriages and gestational weeks of current miscarriages
(mean ± SD) were 2.34 ± 0.77 and 10.16 ± 3.58,
respectively.
The present qRT-PCR analyses were successful for
all samples, and PTX3 mRNA expression in the patient
group was 116-fold higher than that in the control group
(p = 0.0001; Figure 1). In the patient group, no significant
correlations were found between PTX3 mRNA expression
levels and maternal ages, numbers of live births, previous
numbers of miscarriages, and gestational weeks of miscarriage.
The URPL patients were divided into the patients
with no previous live births and the patients with previous
live births, and PTX3 mRNA expression levels did
not differ significantly between the two groups. However,
PTX3 mRNA expression levels of the patients with no live
births were significantly elevated in comparison with PTX3
mRNA expression levels of URPL patients and control
subjects with previous live births (p = 0.0001; Figure 2). Immunohistochemistry analysis showed that the
PTX3 protein expression was widely distributed in the
villous and extravillous cytotrophoblast, and also in the stromal and endothelial cells. Similar tissue distribution
was observed in both URPL patients and control subjects.
The PTX3 protein expression was scored as 1 in four, 2 in
10, 3 in 16 and 4 in 20 URPL patients, whereas the same
scores of 1, 2, 3 and 4 were assigned to 28, 13, seven
and two control subjects, respectively. Figure 3(a) to 3(d)
shows representative images of the IHC staining scores.
As in PTX3 mRNA expression analyses, protein expression
levels differed significantly between URPL patients
and controls (p = 0.0001) [Figure 3(e)]. We also compared
the results of both methodologies, and there was a weak
positive correlation between PTX3 mRNA and protein
expression levels (p = 0.023; r = 0.227).
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