INCREASED EXPRESSION OF PENTRAXIN 3 IN PLACENTAL TISSUES FROM PATIENTS WITH UNEXPLAINED RECURRENT PREGNANCY LOSS
Zeybek S1,*, Tepeli E2, Cetin GO3, Caner V3, Senol H4, Yildirim B2, Bagci G3
*Corresponding Author: Selcan Zeybek, M.D., Department of Medical Genetics, Erzurum Regional Training and Research Hospital, Cat Volu Street, 25070, Erzurum, Turkey. Tel.: +90-506-399-2644. Fax: +90-442-232-5025. E-mail: selcankesan@yahoo.com
page: 21

RESULTS

The characteristics of the URPL patients are shown in Table 2. Patient and control groups did not differ significantly in terms of age (p = 0.819), and 30 (60.0%), 17 (34.0%), and three (6.0%) URPL patients had zero, one, and two live births, respectively. Numbers of previous miscarriages and gestational weeks of current miscarriages (mean SD) were 2.34 0.77 and 10.16 3.58, respectively. The present qRT-PCR analyses were successful for all samples, and PTX3 mRNA expression in the patient group was 116-fold higher than that in the control group (p = 0.0001; Figure 1). In the patient group, no significant correlations were found between PTX3 mRNA expression levels and maternal ages, numbers of live births, previous numbers of miscarriages, and gestational weeks of miscarriage. The URPL patients were divided into the patients with no previous live births and the patients with previous live births, and PTX3 mRNA expression levels did not differ significantly between the two groups. However, PTX3 mRNA expression levels of the patients with no live births were significantly elevated in comparison with PTX3 mRNA expression levels of URPL patients and control subjects with previous live births (p = 0.0001; Figure 2). Immunohistochemistry analysis showed that the PTX3 protein expression was widely distributed in the villous and extravillous cytotrophoblast, and also in the stromal and endothelial cells. Similar tissue distribution was observed in both URPL patients and control subjects. The PTX3 protein expression was scored as 1 in four, 2 in 10, 3 in 16 and 4 in 20 URPL patients, whereas the same scores of 1, 2, 3 and 4 were assigned to 28, 13, seven and two control subjects, respectively. Figure 3(a) to 3(d) shows representative images of the IHC staining scores. As in PTX3 mRNA expression analyses, protein expression levels differed significantly between URPL patients and controls (p = 0.0001) [Figure 3(e)]. We also compared the results of both methodologies, and there was a weak positive correlation between PTX3 mRNA and protein expression levels (p = 0.023; r = 0.227).



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