ASSESSMENT OF GENOTOXICITY OF VINCRISTINE, VINBLASTINE AND VINORELBINE IN HUMAN CULTURED LYMPHOCYTES: A COMPARATIVE STUDY
Mhaidat NM, Alzoubi KH, Khabour OF, Alawneh KZ, Raffee LA, Alsatari ES, Hussein EI, Bani-Hani KE
*Corresponding Author: Nizar M. Mhaidat, Ph.D., Department of Clinical Pharmacy, Faculty of Pharmacy, Jordan University of Science and Technology, Amman-Ramtha Hwy, Irbid 22110, Jordan. Tel: +962- 2-720-100. Fax: +962-2-720-1075. E-mail: nizarm@just.edu.jo
page: 13

RESULTS

Figure 1 shows the level of 8-OHdG in all groups. Levels of 8-OHdG in drug-treated groups (VCR, VBL and VRL; each at concentrations of 0.01 and 0.1 μg/mL) were higher than those in the control group (p <0.05). These results indicate that VCR, VBL and VRL at concentrations of 0.01 and 0.1 μg/ mL induce oxidative DNA damage. Figure 1 also shows that at both tested concentrations, the magnitude of the increase in 8-OHdG was higher in the VBL-treated group compared to the VCR and VRL groups (Figure 1). Sister chromatid exchanges were observed in differentially stained M2 metaphase cells that have 42-48 chromosomes. There was an increase in SCEs frequency between control and drug-treated groups (VCR, VBL and VRL, each at concentrations of 0.01 and 0.1 μg/mL). However, this increase did not reach a significant level (p >0.05) (Figure 2). Previous studies showed that Vinca alkaloid drugs affect spindle fibers and causes cell arrest in the mitotic phase. As expected, the mitotic index was higher in the VCR-, VBL- and VRL-treated groups compared to the control group (Figure 3). In addition, in all treated groups, the effect was dose dependent. Moreover, the magnitude of mitotic arrest was similar in VCR, VBL and VRL (Figure 3).



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