NOVEL PATTERNS OF THE EPSTEIN-BARR NUCLEAR
ANTIGEN (EBNA-1) V-VAL SUBTYPE IN EBV-ASSOCIATED
NASOPHARYNGEAL CARCINOMA FROM VIETNAM
Thuan LD1, Kha ND2, Minh NT3, Thuy LHA1,*
*Corresponding Author: Thuy Le Huyen Ai, Ph.D., Associate Professor, Department of Pharmaceutical
and Medical Biotechnology, Faculty of Biotechnology, Room 304, 97 Vo Van Tan Street, Ward 6, District 3,
Ho Chi Minh City Open University, Ho Chi Minh City, Vietnam.
Tel: +84-905-784-471. E-mail: thuy.lha@ ou.edu.vn
page: 61
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Abstract
The Epstein-Barr nuclear antigen 1 (EBNA-1) gene,
plays a key role in viral infection, immortalization, viral
genome replication, transcription and maintenance, and
is the frequently detected gene, protein in both latent and
lytic stage of Epstein-Barr virus (EBV). Based on the
amino acid at position 487, EBNA-1 was classified into
five subtypes, including P-Ala, P-Thr, V-Val, V-Pro and
V-Leu. In Vietnam, an Asian country with a high incidence,
mortality rates of nasopharyngeal carcinoma (NPC), had
limited research on the EBNA-1 variation. Therefore, the
aim of the current study was to identify the pattern of the
EBNA-1 V-Val subtype in Vietnamese NPC patients, for
its value further applied in NPC patients. Fifty-eight NPC
biopsy samples were collected from local patients, analyzed
by nested-polymerase chain reaction (nested-PCR),
sequencing and compared to a previous B95-8 prototype
sequence. Four EBNA-1 subtypes, including V-Val (35/44,
79.55%), P-Ala (2/44, 4.55%), P-Thr (5/44, 11.36%), and
V-Leu (2/44, 4.55%), were observed in 44/58 samples.
The sequences of the V-Val subtype were compared to the
B95-8 prototype, resulting in five patterns, contained seven
consensus changes, including five amino acid changes at
positions 487, 499, 502, 524, 594, and two silent changes at
residues 520 and 553. Of these, four of five, patterns were
identified as novel patterns of the V-Val subtype, showing
the different changes of amino acids at positions 492, 528,
529, 553, 585 and 588, by comparison with previous studies
of V-Val EBNA-1. Those data suggested the profile of variation
patterns of the EBNA-1 gene, related to geographic
distribution, in Vietnamese NPC patients.
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