OP22. TRANSCRIPTIONAL REGULATION OF HUMAN SOX18 GENE - THE ROLE OF SP1 TRANSCRIPTION FACTOR
I, PETROVIC, M, Stevanovic Institute of Molecular Genetics and Genetic Engineering e-mail: hmgbox@eunet.yu
*Corresponding Author:
page: 45

Abstract

Nowadays, it is well known that SOX gene family has important role in the decision of cell fates during early development. Among other members, we are focused on analysis of transcriptional regulation of human SOX18 gene. This gene is expressed in a wide range of fetal and adult tissues indicating its function is not restricted to early development. Human SOX18 gene encodes transcription factor that plays a role in hair, blood vessel, lymphatic vessel development and vascular cell growth. The aim of our study has been to determine and characterize the promoter of the human SOX18 gene and to elucidate molecular mechanisms underlying the regulation of its expression. We have isolated and performed the first characterization of human SO X18 promoter. We have identified the transcription start point and carried out the structural and functional analysis of the regulatory region responsible for SOX18 expression in HeLa cell line. Using promoter-reporter constructs, we have determined the minimal SOX18 promoter region that shows basal promoter activity. Further, we have investigated functional properties of GC-rich motif inside the core promoter sequence. Sp1 is common transcription factor that binds within GC-rich motifs, often present in TATA-less promoters, like SOX18 promoter. Our experiments confirmed binding of Sp1 transcription factor to proximal GC-rich motif. Finally, functional analysis revealed that over expression of Sp1 transcription factor in HeLa cell line positively regulates the activity of SOX18 promoter and in vivo expression of SOX18 gene.




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