OP21. STRUCTURAL AND FUNCTIONAL CHARACTERIZATION OF THE HUMAN SOX3 PROMOTER
M, MOJSIN, N, Kovačević-Grujičić, A, Krstić and M, Stevanović Institute for Molecular Genetics and Genetic Engineering, Vojvode Stepe 444a, P.O. Box 23, 11010 Belgrade, Serbia and Montenegro e-mail: mojsin@eunet.yu
*Corresponding Author:
page: 44

Abstract

Family of SOX genes encodes proteins that appear to govern cell fate decisions during embryogenesis by functioning both as classical transcription factors and architectural components of chromatin. Sox3, an X-linked member of the family, is expressed in the CNS from the earliest stages of development. It is considered to be one of the earliest neural markers in vertebrates playing the role in specifying neuronal fate. The aim of this study has been to determine and characterize the promoter of the human SOX3 gene and to elucidate molecular mechanisms underlying the regulation of its expression. In this study we hav e isolated and performed the first characterization of the human SOX3 promoter. We have identified the transcription start point and carried out the structural and functional analysis of the regulatory region responsible for SOX3 expression in NT2/D1 cell line. Using promoter-reporter constructs we have determined the minimal SOX3 promoter region that confers the basal promoter activity, as well as, two regulatory elements which have the positive effects on the promoter activity. We have investigated the properties of TATA box and three conserved motifs within the core promoter sequence that bind transcription factors Sp1, USF and NF-Y, and confirmed their functional relevance for constitutive SOX3 expression in NT2/D1 cells.




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