RENOPATHOLOGICAL MICROSTRUCTURE VISUALIZATION
FROM FORMALIN FIXED KIDNEY TISSUE BY MATRIXASSISTED
LASER/DESORPTION IONIZATION-TIME-OFFLIGHT
MASS SPECTROMETRY IMAGING
Fröhlich S1, Putz B1, Schachner H2, Kerjaschki D2,Allmaier G1, Marchetti-Deschmann M1,*
*Corresponding Author: Dr. Martina Marchetti-Deschmann, Vienna University of Technology, Institute
of Chemical Technologies and Analytics, Getreidemarkt 9/164-IAC, 1060 Vienna, Austria; Tel.: +43-1-
58801-15152; Mobile: +43+664-605887663; Fax: +43-1-58801-915162; E-mail: martina.marchettideschmann@
tuwien.ac.at
page: 13
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Abstract
Understanding early stage renal malfunctions
with regard to the glomerular filtration processes
is essential for nephropathological prescreening
strategies and intervention at an early stage. Mass
spectrometry imaging (MSI) in combination with
histopathology can provide an universal analytical
approach. Proteomic and lipidomic aspects of glomerular
biocompositions were applied for microstructural
differentiation in healthy rat kidney samples.
Usability of commonly used tissue embedding
media and the compatibility of histological staining
and fixation methods were of interest. It was demonstrated
that ultra-thin tissue samples (500 nm, 1
and 10 mm) can be used for lipid and peptide-based
differentiation at the glomerular resolution level in
formalin-fixed tissue samples in combination with
preceding histological staining for correlating optical
and molecular mass images.
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