RENOPATHOLOGICAL MICROSTRUCTURE VISUALIZATION
FROM FORMALIN FIXED KIDNEY TISSUE BY MATRIXASSISTED
LASER/DESORPTION IONIZATION-TIME-OFFLIGHT
MASS SPECTROMETRY IMAGING
Fröhlich S1, Putz B1, Schachner H2, Kerjaschki D2,Allmaier G1, Marchetti-Deschmann M1,*
*Corresponding Author: Dr. Martina Marchetti-Deschmann, Vienna University of Technology, Institute
of Chemical Technologies and Analytics, Getreidemarkt 9/164-IAC, 1060 Vienna, Austria; Tel.: +43-1-
58801-15152; Mobile: +43+664-605887663; Fax: +43-1-58801-915162; E-mail: martina.marchettideschmann@
tuwien.ac.at
page: 13
download article in pdf format
|
|
Abstract
Understanding early stage renal malfunctions
with regard to the glomerular filtration processes
is essential for nephropathological prescreening
strategies and intervention at an early stage. Mass
spectrometry imaging (MSI) in combination with
histopathology can provide an universal analytical
approach. Proteomic and lipidomic aspects of glomerular
biocompositions were applied for microstructural
differentiation in healthy rat kidney samples.
Usability of commonly used tissue embedding
media and the compatibility of histological staining
and fixation methods were of interest. It was demonstrated
that ultra-thin tissue samples (500 nm, 1
and 10 mm) can be used for lipid and peptide-based
differentiation at the glomerular resolution level in
formalin-fixed tissue samples in combination with
preceding histological staining for correlating optical
and molecular mass images.
|
|
|
|
 |
Number 21
VOL. 21, 2018 Accepted articles (Accepted, unedited articles, published online and can be cited. The final edited and printed version of the manuscript will appear in future) |
Number 21
VOL. 21, 2018 Supplement |
Number 20
VOL. 20 (2), 2017 |
Number 20
VOL. 20 (1), 2017 |
Number 19
VOL. 19 (2), 2016 |
Number 19
VOL. 19 (1), 2016 |
Number 18
VOL. 18 (2), 2015 |
Number 18
VOL. 18 (1), 2015 |
Number 17
VOL. 17 (2), 2014 |
Number 17
VOL. 17 (1), 2014 |
Number 16
VOL. 16 (2), 2013 |
Number 16
VOL. 16 (1), 2013 |
Number 15
VOL. 15 (2), 2012 |
Number 15
VOL. 15, 2012 Supplement |
Number 15
Vol. 15 (1), 2012 |
Number 14
14 - Vol. 14 (2), 2011 |
Number 14
The 9th Balkan Congress of Medical Genetics |
Number 14
14 - Vol. 14 (1), 2011 |
Number 13
Vol. 13 (2), 2010 |
Number 13
Vol.13 (1), 2010 |
Number 12
Vol.12 (2), 2009 |
Number 12
Vol.12 (1), 2009 |
Number 11
Vol.11 (2),2008 |
Number 11
Vol.11 (1),2008 |
Number 10
Vol.10 (2), 2007 |
Number 10
10 (1),2007 |
Number 9
1&2, 2006 |
Number 9
3&4, 2006 |
Number 8
1&2, 2005 |
Number 8
3&4, 2004 |
Number 7
1&2, 2004 |
Number 6
3&4, 2003 |
Number 6
1&2, 2003 |
Number 5
3&4, 2002 |
Number 5
1&2, 2002 |
Number 4
Vol.3 (4), 2000 |
Number 4
Vol.2 (4), 1999 |
Number 4
Vol.1 (4), 1998 |
Number 4
3&4, 2001 |
Number 4
1&2, 2001 |
Number 3
Vol.3 (3), 2000 |
Number 3
Vol.2 (3), 1999 |
Number 3
Vol.1 (3), 1998 |
Number 2
Vol.3(2), 2000 |
Number 2
Vol.1 (2), 1998 |
Number 2
Vol.2 (2), 1999 |
Number 1
Vol.3 (1), 2000 |
Number 1
Vol.2 (1), 1999 |
Number 1
Vol.1 (1), 1998 |
|
|
|
