OP03. GENOMIC PROFILING OF OVARIAN CANCER FOR CHROMOSOME 17 BY ARRAY CGH ANALYSIS
DIMOVA I1, Orsetti B2, Rouge C2, Ursule L2, Lasorsa L2, Dimitrov R3, Doganov N3, Toncheva D1, Theillet Ch2 1 Department of Medical Genetics, Medical University - Sofia, Bulgaria 2 Genotype et Phenotype Tumoraux, EMI229 INSERM/Universite Montpellier I, CRLC Val D'Aurelle - Paul Lamarque, Montpellier, France 3 Clinics of Operative Gynecology, University Hospital of Obstetrics and Gynecology - Sofia, Bulgaria email: dragatoncheva@yahoo.com
*Corresponding Author:
page: 36

Abstract

We analyzed genomic imbalances affecting chromosome 17 by array-CGH in 28 primary ovarian cancers and 9 ovarian cancer cell lines, using a home made BAC-array covering this chromosome at a mean density of 1 BAC clone/0.8 Mb. We identified regions of highly frequent gains or losses, since they affected more than 40% of ovarian cancers and determined sites showing alterations of elevated amplitude (amplifications or homozygous deletions). Doing this we also identified at least two adjacent changed clones. Finally, we characterized the smallest regions of overlap for gains. This allowed us to determine anomalies strongly associated to the disease such as: deletions at 17p12-13 or amplifications at 17q12, 17q23.2, 17q24.3. The resolution of array-CGH gives an almost direct link to the human genome sequence and, hence, to a list of candidate genes. Our results defined more precisely the gains in 17q12-q24, finding as strong candidates for ovarian carcinogenesis the ge nes LASP1 (17q12), TGF11 (17q21.32), MUL (17q23.2), TBX2 (17q23.2), AXIN2 (17q24.3) and GRB2 (17q25.1). We were interested in verifying whether some of the anomalies we had identified were diagnostic in terms of separating tumors of different histological types or stage. We performed two comparisons: (1) serous and non-serous; (2) early and late stage ovarian tumors. To identify alterations, specific of a particular histological and stage group, we determined regions whose alterations was at least 40% more frequent in one group compared to another. We found that gains of 17q11.2-12 and 17q25 are associated with non-serous histology. Gains of 17q23.2 and 17q24.3 and losses of 17p and 17q were specific for late stage ovarian cancers.




Number 26
Number 26 VOL. 26(2), 2023 All in one
Number 26
VOL. 26(2), 2023
Number 26
VOL. 26, 2023 Supplement
Number 26
VOL. 26(1), 2023
Number 25
VOL. 25(2), 2022
Number 25
VOL. 25 (1), 2022
Number 24
VOL. 24(2), 2021
Number 24
VOL. 24(1), 2021
Number 23
VOL. 23(2), 2020
Number 22
VOL. 22(2), 2019
Number 22
VOL. 22(1), 2019
Number 22
VOL. 22, 2019 Supplement
Number 21
VOL. 21(2), 2018
Number 21
VOL. 21 (1), 2018
Number 21
VOL. 21, 2018 Supplement
Number 20
VOL. 20 (2), 2017
Number 20
VOL. 20 (1), 2017
Number 19
VOL. 19 (2), 2016
Number 19
VOL. 19 (1), 2016
Number 18
VOL. 18 (2), 2015
Number 18
VOL. 18 (1), 2015
Number 17
VOL. 17 (2), 2014
Number 17
VOL. 17 (1), 2014
Number 16
VOL. 16 (2), 2013
Number 16
VOL. 16 (1), 2013
Number 15
VOL. 15 (2), 2012
Number 15
VOL. 15, 2012 Supplement
Number 15
Vol. 15 (1), 2012
Number 14
14 - Vol. 14 (2), 2011
Number 14
The 9th Balkan Congress of Medical Genetics
Number 14
14 - Vol. 14 (1), 2011
Number 13
Vol. 13 (2), 2010
Number 13
Vol.13 (1), 2010
Number 12
Vol.12 (2), 2009
Number 12
Vol.12 (1), 2009
Number 11
Vol.11 (2),2008
Number 11
Vol.11 (1),2008
Number 10
Vol.10 (2), 2007
Number 10
10 (1),2007
Number 9
1&2, 2006
Number 9
3&4, 2006
Number 8
1&2, 2005
Number 8
3&4, 2004
Number 7
1&2, 2004
Number 6
3&4, 2003
Number 6
1&2, 2003
Number 5
3&4, 2002
Number 5
1&2, 2002
Number 4
Vol.3 (4), 2000
Number 4
Vol.2 (4), 1999
Number 4
Vol.1 (4), 1998
Number 4
3&4, 2001
Number 4
1&2, 2001
Number 3
Vol.3 (3), 2000
Number 3
Vol.2 (3), 1999
Number 3
Vol.1 (3), 1998
Number 2
Vol.3(2), 2000
Number 2
Vol.1 (2), 1998
Number 2
Vol.2 (2), 1999
Number 1
Vol.3 (1), 2000
Number 1
Vol.2 (1), 1999
Number 1
Vol.1 (1), 1998

 

 


 About the journal ::: Editorial ::: Subscription ::: Information for authors ::: Contact
 Copyright © Balkan Journal of Medical Genetics 2006