INVOLVEMENT OF CHROMOSOMES 7, 18 AND X IN MITOMYCIN C-INDUCED MICRONUCLEI
Hovhannisyan GG1,2*, Mkrtchyan H1,2, Liehr T2, Aroutiounian R1
*Corresponding Author: Dr. Galina G. Hovhannisyan, Department of Genetics and Cytology, State University, Biological Faculty, 1 Alex Manoukian Street, Yerevan 375025, Armenia; Tel.: +374-10552354; Fax: +374-10554641; e-mail: hovgalina@list.ru
page: 45
download article in pdf format

Abstract

The involvement of specific chromosomes in the formation of micronuclei (MN) is neither well studied nor understood. We studied the involvement of chromosomes 7, 18 and X in mitomycin C (MMC)-induced MN. A total of 1,202 female MN were studied by three-color fluorescence in situ hybridization (FISH) using centromere-specific probes. After a second round of hybridization, using whole chromosome painting probes, 916 MN were evaluated. The frequency of MN containing whole chromosome painting signals for chromosomes X in 916 male MN was analyzed. Different frequency of involvement of selected chromosomes in MN formation was revealed. Chromosome interphase position, size and inactive (heterochroma­tinized) state of X chromosome in females was discussed as possible reasons of their non random involvement in MN formation. There was no clear relationship between chromosome interphase position and size and migration in MN. However, it was shown that X-chromosomal material was over represented in female- and under represented in male-derived MN. We speculated about a preferred inclusion of the inactive female X-chromosome into MN.

 

      Keywords: Chromosomes 7, 18, X; Fluorescence in situ hybridization (FISH); Mitomycin C (MMC); Micro­nuclei (MN)

 

__________________________________________________

            1 Department of Genetics and Cytology, State University, Biological Faculty,Yerevan, Armenia

            2 Institute of Human Genetics and Anthropology, Jena, Germany

 




Number 19
VOL. 19 (2), 2016
Number 19
VOL. 19 (1), 2016
Number 18
VOL. 18 (2), 2015
Number 18
VOL. 18 (1), 2015
Number 17
VOL. 17 (2), 2014
Number 17
VOL. 17 (1), 2014
Number 16
VOL. 16 (2), 2013
Number 16
VOL. 16 (1), 2013
Number 15
VOL. 15 (2), 2012
Number 15
VOL. 15, 2012 Supplement
Number 15
Vol. 15 (1), 2012
Number 14
14 - Vol. 14 (2), 2011
Number 14
The 9th Balkan Congress of Medical Genetics
Number 14
14 - Vol. 14 (1), 2011
Number 13
Vol. 13 (2), 2010
Number 13
Vol.13 (1), 2010
Number 12
Vol.12 (2), 2009
Number 12
Vol.12 (1), 2009
Number 11
Vol.11 (2),2008
Number 11
Vol.11 (1),2008
Number 10
Vol.10 (2), 2007
Number 10
10 (1),2007
Number 9
1&2, 2006
Number 9
3&4, 2006
Number 8
1&2, 2005
Number 8
3&4, 2004
Number 7
1&2, 2004
Number 6
3&4, 2003
Number 6
1&2, 2003
Number 5
3&4, 2002
Number 5
1&2, 2002
Number 4
Vol.3 (4), 2000
Number 4
Vol.2 (4), 1999
Number 4
Vol.1 (4), 1998
Number 4
3&4, 2001
Number 4
1&2, 2001
Number 3
Vol.3 (3), 2000
Number 3
Vol.2 (3), 1999
Number 3
Vol.1 (3), 1998
Number 2
Vol.3(2), 2000
Number 2
Vol.1 (2), 1998
Number 2
Vol.2 (2), 1999
Number 1
Vol.3 (1), 2000
Number 1
Vol.2 (1), 1999
Number 1
Vol.1 (1), 1998

 

 


 About the journal ::: Editorial ::: Subscription ::: Information for authors ::: Contact
 Copyright © Balkan Journal of Medical Genetics 2006