EFFECTS OF β-CAROTENE AND PORPHYRIDIUM CRUENTUM POLYSACCHARIDE EXTRACT ON MITOMYCIN C SENSITIVITY OF BLOOD LYMPHOCYTE CHROMOSOMES OF RADIOLOGY TECHNICIANS
Senol S1,2, Pehlivan M3, Ozdemir G1, Conk-Dalay M4, Kucuk O5, Pehlivan S1,6*
*Corresponding Author: Sacide Pehlivan, PhD., Genetic Diseases Research and Application Center, Ege University, 35100, Izmir, Turkey; Tel.: +90-232-388-0110/2856; Fax: +90-232-388-1036; E-mail: psacide@ hotmail.com, pehlivan@gantep.edu.tr
page: 63

Abstract

We have investigated the individual and combined effects of β-carotene (βC) and a crude polysaccharide extract of the red alga Porphyridium cruentum (PC) on Mitomycin C (Mit-C) sensitivity of peripheral blood lym­phocytes of humans occupationally exposed to low dose gamma radiation. Peripheral blood lymphocytes in vitro were treated with Mit-C and the chromosome abnormal­ities in the presence or absence of βC and/or PC were recorded. The incidence of chromosomal abnormalities increased from 1.9% in cell cultures without Mit-C to 7.8% in those with Mit-C with a significant increase in chromosomal aberrations (chromatid breaks, chromosome breaks, dicentrics and rings) (p <0.05). However, in Mit-C treated cultures that contained βC+PC the incidence was 1.3%. Mitomycin C-induced aberrations in the lympho­cytes decreased significantly with the addition of βC and PC to the culture. This suggests that mutagen sensitivity may be reduced by a βC+PC combination and that this combination may protect humans exposed to radiation.

      Key words: Lymphocyte; Mitomycin C (Mit-β); Porphyridium cruentum (PC); β-Carotene (βC); X-rays; Chromosome aberration(s).

 

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        1 Department of Biology, Faculty of Science, Ege Univer­sity, Izmir, Turkey

        2 Department of Internal Medicine, Ataturk Education and Research Hospital, Izmir, Turkey

        3 Department of Internal Medicine, Medical Faculty, Gaziantep University, Gaziantep, Turkey

        4 Department of Bioengineering, Faculty of Engineering, Ege University, Izmir, Turkey

        5 Karmanos Cancer Institute, Wayne State University, Detroit, Michigan, USA

        6 Genetic Diseases Research and Application Center, Ege University, Izmir, Turkey

 

 




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