PP173. ETHICAL ISSUESS OF INTERPRETATION A FINAL REPORT IN DNA IDENTIFICATION - CASE STUDY
KOVACEVIC LEJLA Bakal Narcisa Marjanovic Damir Laboratory for forensic genetic, Institute for Genatic Engineering and Biotechnology, University of Sarajevo, Bosnia and Herzegovina e-mail: lejla.kovacevic@ingeb.ba; narcisa.bakal@ingeb.ba; damir.marjanovic@ingeb.ba
*Corresponding Author:
page: 173

Abstract

Over the past decades, a forensic technology has entered into common use. At the present, DNA typing of biological materials has become one of the most powerful tools for personal identification, paternity testing and criminal investigations. The biological material to be examined in DNA analysis fluctuates from liquid blood to dried blood stains, minor amounts of epithelia, hair shafts, skeletal remains/ bones, dried sperm etc. Nowadays, DNA typing is based upon variability of up to 16 noncoding DNA stretches in the human genome referred as STR. Analysis reported in this presentation includes comparison of obtained DNA profile from recovered skeletal remains with nuclear DNA profiles of potential parents, which provided their blood samples. Blood samples are extracted using standard Qiage n method and for bone sample optimized Qiagen method was applied. The PowerPlex 16 kit was employed in simultaneously amplification of 15 STR loci on GeneAmp PCR Thermocycler 9700. Electrophoresis of the amplified products has been performed on an ABI PRISM 377 genetic analyzer. DNA parental profiles are compared with profile generated from skeletal remains by routine paternity testing method. Maternity was completely positive, but following paternity test showed that obligate alleles of skeletal remains did not match to potential father's alleles at the 6 STR. Consequently potential father is excluded as a biological. This DNA identification case was extremely challenging in making a final report because of bioethical question that was arisen from it: How to make a final report and confirm identity only by maternal line and not by paternal.




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