PP109. ASSOCIATION ANALYSIS OF THE INTERFERON-GAMMA GENE POLYMORPHISM WITH ALLERGIC RHINITIS IN VOLGA-URAL REGION OF RUSSIA
A. KHUZINA1, A. Karunas1, A. Biktasheva2, A. Yuldasheva3, E. Etkina2, E. Khusnutdinova1 1. Institute of Biochemistry and Genetics, Ufa Scientific Center, Russian Academy of Sciences, Russia, 2. Bashkir Medical State University, Russia 3. Pediatric polyclinic N1, Ufa, Russia e-mail: Hiame@mail.ru
*Corresponding Author:
page: 97

Abstract

Allergic rhinitis (AR) is an inflammatory disease of the nasal mucosa that is characterized by typical clinical symptoms such as sneezing, nasal congestion, and watery rhinorrhea. It is the most common form of atopic disease, affecting about 10-25% population in the world. Inflammatory reactions in allergic rhinitis are regulated by many cytokines. Interferon-gamma (IFNG) plays an important role in modulating almost all phases of the immune response. The DNA sequence of the human IFNG gene shows the presence of a variable-length CA repeat in the first intron of the gene. The purpose of the present study was to investigate the allele and genotype distribution of this microsatellite locus in patients with allergic rhinitis and healthy donors from Volga-Ural region of Russia. The patient group consisted of 220 individuals with allergic rhinitis, the control group included 188 unrelated non-allergic individuals with different ethnic origins (Russians, Tatars, Bashkirs). Genomic DNA was extracted from peripheral blood leucocytes by standard phenol/chloroform method. Genotyping was performed by polymerase chain reaction with specific primers. No significant differences in allele or genotype frequencies between AR patients and healthy donors were observed (p>0,05). The 11 various genotypes are revealed, most common was genotype *12/*13, determined in the patients in 37,73 % of cases, in the control group - 44,68%. The frequency of genotype *13/*13 was 18,18% in the patients and 13,30 % - in the control. The alleles *12 and *13 were prevalent in both compared groups, revealed in 42,05% and 42,95 % of cases in AR patients, 46,81 % and 41,76 % - in the control group, respectively. The distribution of allele frequencies of this microsatellite locus was similar to the European populations (Pociot F., 1997). Thus, the analysis of CA repeat in the first intron of the IFNG gene has not revealed significant differences between AR patients and healthy donors from Volga-Ural region of Russia.

 




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