PP36. EFFECT OF HIGHT-GLUCOSE CONCENTRATION ON THE EXPRESSION OF TYPE I AND III COLLAGEN IN CULTURED HUMAN SKIN FIBROBLASTS
ANDREEA IREN SERBAN1, E. Condac2, Anca Dinischiotu2, Marieta Costache2 1 University of Agricultural Sciences and Veterinary Medicine Bucharest, Faculty of Veterinary Medicine, 105 Spl. Independentei, Bucharest 5, 050095, Romania; 2 University of Bucharest, Molecular Biology Center, 91-95 Spl. Independentei, Bucharest 5, 050095, Romania e-mail: irensro@yahoo.com
*Corresponding Author:
page: 62

Abstract

Extracellular matrix macromolecules such as collagen and fibronectin are progressively altered during aging and diabetes. A number of pathways are activated in the hyperglycemic milieu and in diabets, such as the aldose reductase pathway (AR), activation of protein kinase C (PKC), especially the β isoform, and the generation of advanced glycation end products (AGE). Activation of these pathways has been demonstrated in glucose-treated glomeruli or mesangial cells.

In the present study, we explored the effect of high glucose levels, mimicking diabetic conditions, on the expression of type I and III collagen, the most abundant matrix protein, in cultured human skin fibroblasts. Total RNA was extracted from human dermal fibroblasts incubated with varying amounts of glucose: 1 g/l (normoglycemic), 2, 4 and 6 g/l (hyperglycemic) D-glucose for 24 hours and mRNA expression of type I and III collagen was analyzed by quantitative real-time PCR.

Incubation of human dermal fibroblasts with high glucose concentration resulted in a significant increase of procollagen-α1 (I) mRNA collagen type I expression in a dose-dependent manner whereas expression of collagen III was unaffected.

Our results suggest that high level of glucose may influence the process of skin aging through mild stimulation of collagen type I synthesis and possible of other extracellular matrix proteins. The change in the ratio of collagen I /collagen III expression and the increase of the synthesis of collagen contributes to a decreased tensile strength and mechanical stability of both the connective tissues and the induced scar tissue in patience with diabetes.




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