ASSOCIATION OF THE MMP7 181A>G PROMOTER POLYMORPHISM WITH EARLY ONSET OF CHRONIC OBSTRUCTIVE PULMONARY DISEASE
Tacheva T, Dimov D, Anastasov A, Zhelyazkova Y, Kurzawski M, Gulubova M, Drozdzik M, Vlaykova T
*Corresponding Author: Assistant Professor Tanya Tacheva, Department of Chemistry and Biochemistry, Medical Faculty, Trakia University, 11 Armeiska Str., Stara Zagora, Bulgaria. Tel: +359878334176. E-mail: tanya.ta4eva@abv.bg
page: 4

RESULTS

The PCR product amplified with the primers for MMP7 181A>G was 150 bp in length. The EcoRI digestion resulted in 150 bp for the AA genotype, 150, 120 and 30 bp for the AG genotype, and 120 and 30 bp for the GG genotype (Figure 1). The genotype distribution in both controls and patients did not deviate from HWE (p = 0.998 and p = 0.999). After statistical processing of the data, we found no differences in the genotype and allele distribution of the MMP7 181A>G polymorphism between COPD patients and controls (p = 0.341 and p = 0.214) (Table 2). In the group of patients, there was no association between the genotype and gender (p = 0.358), smoking habit (p = 0.587), stage of the disease (p = 0.924) or spirometric indexes for lung function (for FEV1: p = 0.598 and for FEV1/FVC: p = 0.3, Kruskal-Wallis test). Association of the MMP-7 G allele with severity of COPD was also not found (p = 0.876). However, the carriers of the G allele (AG and GG genotypes) appeared to develop COPD significantly early compared to those with the AA genotype (61.01 10.11 vs. 64.87 9.00 years, p = 0.032, Student t-test). When the genotype distribution was studied only in the patients (n = 76) and controls (n = 106) younger than 60 years, significant difference was found. The frequency of the carriers of the G allele (AG and GG genotypes), was higher in COPD patients than in controls, showing a 3-fold higher risk for the development of COPD before the age of 60 years (Table 3).



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